1 / 18

Investigating the Anti-fungal Properties of Subterranean Termites

Investigating the Anti-fungal Properties of Subterranean Termites . Yasamin Sharifzadeh , Katie Gorick , Wong Hong Jie , Samuel Lau. Background . Our project. Objectives Hypothesis Overview of project. Objectives. Overview. American vs Asian. Hypothesis.

brede
Download Presentation

Investigating the Anti-fungal Properties of Subterranean Termites

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Investigating the Anti-fungal Properties of Subterranean Termites YasaminSharifzadeh, Katie Gorick, Wong Hong Jie, Samuel Lau

  2. Background

  3. Our project Objectives Hypothesis Overview of project

  4. Objectives

  5. Overview American vs Asian

  6. Hypothesis

  7. Methodology Body fluid extraction (Total system) Hemolymph extraction Hemolymph w/o protozoa and bacteria Hemolymph w/ bacteria but no protozoa Anti-fungal assay

  8. Body fluid extraction (Total system)

  9. Body fluid extraction (Total system) • Wash the termites in a beaker • Filter termites with a sieve and repeat washing for 2 more times • Crush the termites with a homogenizer • Homogenize using the phosphate buffer solution • Centrifuge to pellet tissue debris • Collect the supernatant • Place the supernatant in micro centrifuge tubes • Centrifuge at 1300rpm for 10 minutes • The body fluids are now separated • Extract the supernatant and filter it with a micro-filter

  10. Hemolymph extraction

  11. Hemolymph extraction

  12. Hemolymph extraction (cont’d)

  13. Hemolymph (cont’d)

  14. Anti-fungal assay

  15. Anti-fungal assay

  16. Anti-fungal assay

  17. Anti-fungal assay

  18. Timeline

More Related