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Chapter 9 How Cells Reproduce. Start with 3 questions: What kind of information guides inheritance? How is the information copied in a parent cell before being passed to the daughter cell? What kind of mechanisms actually parcel out info to daughter cells?. 9-1 Overview.
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Chapter 9 How Cells Reproduce • Start with 3 questions: • What kind of information guides inheritance? • How is the information copied in a parent cell before being passed to the daughter cell? • What kind of mechanisms actually parcel out info to daughter cells?
9-1 Overview • Mitosis – nuclear division in somatic cells • Growth, replacing cells, tissue repair • Plants, animals, fungi, protists • Meiosis – formation of gametes/spores • Basis of sexual reproduction • Develop from germ cells • Prokaryotes? • Binary fission (p. 336)
Chromosomes • Characteristic number • Orderly coiling • DNA winds 2x around histones to make nucleosomes • Centromere • Kinetochore
Nuclear DNA one chromosome (threadlike and now duplicated; two DNA molecules + proteins) one chromosome (one dispersed DNA molecule + proteins; not duplicated) one chromosome (duplicated and also condensed tightly) p.61
9.2 Cell Cycle • Series of events from one cell division to the next
Interphase • G1 • Growth/functional • S • Synthesis of DNA • G2 • Prepare for division
Mitosis and C-some # • Diploid • 2n • 2 of each type
9.3 Closer look • Prophase • C-somes visible • Centrioles duplicate • Nuclear envelope • MT docks at kinetochores
Metaphase • Alignment of c-somes between spindle poles
Anaphase • Sister chromatids move to opposite spindle poles by motor proteins
Telophase • C-somes reach poles • Decondense • Vesicles reform envelope
9.4 Cytokinesis Plants New fibers made before prophase Vesicles from Golgi fuse and deposit materials for cell plate • Animals • Contractile ring • Cleavage furrow
Identifying phases of Mitosis Phase Determination
9.5 When Control is lost • Cell cycle checkpoints • Proteins monitor DNA structure • Proteins monitor proceeding phases • Favorable conditions? • Kinases • Growth factors
Architecture of control system • Growth assessed at G1 checkpoint • DNA replication success assessed at G2 • Mitosis assessed at M checkpoint
Molecular Mechanisms • Cyclin dependent protein kinases (Cdks) • Cyclins – bind to Cdks, allow them to work as enzymes • G2 - MPF • Growth factors – stimulate • Usually everyone gets equal amounts • Specific cell surface receptors • Broad or specific
Tumor supressors • Inhibit • Prevent binding of cyclins to CdKs • Recessive • Gene p53 – role in G1 checkpoint, checks for DNA errors • Repair or not Figure 2: p53 re-enforces G1 and G2 cell cycle arrest after DNA damage through the cyclin-dependent kinase inhibitor p21WAF1/CIPI Mdm2 and Bax are other p53 transcriptional targets, with Mdm2 regulating p53 levels and Bax mediating apoptosis
Proto-oncogenes • Stimulate • Mutation = oncogene • Dominant • Changes in surface receptors Figure 2. A modified receptor. Under normal circumstances membrane-bound receptors require the binding of their ligand to be in an activated state. In contrast, receptors encoded by oncogenes do not require the regulatory step of ligand binding to be active.
Cancers • 4 characteristics • Grow & divide abnormally • Cytoplasm & membrane altered • Weakened capacity for adhesion • Lethal effects
Control of the cell cycle game http://nobelprize.org/educational_games/medicine/2001/