Homework

1. Homework • Due today – review notes and re-read if necessary. • Test on Friday (tomorrow) on all material covered up to this point. • Create a one page “cheat sheet” which you can bring into the test on Friday. If you forget to do it then no deal for the test. Pull that out now • I will cover the lab write up before we do the lab.

2. Winogradsky Lab • Please sit two people per table with your pre-lab report • What is the purpose of the lab: • To explore the existence of micro-bacteria found within mud samples and determine if contact with light 24 hours a day will produce evidence of colored micro-organisms.

3. Winogradsky Lab • For the procedure, watch the short video and make note of the changes on your pre-lab report: • For example: • 1. We are not using Calcium carbonate, we are replacing it with egg yoke.

4. Procedure • Get a container to mix mud • With a gloved hand, scoop out an amount of mud, then add the yoke of one egg and some shredded papers (about 12) and then add enough water to make it soupy. • Use the top of the soda bottle as a funnel and place the mud, egg yoke & papers into the Mason jar. • Top off with a little water, then seal with the parafilm. • Label with your name and place on bottom shelf.

5. Winogradsky Columns • In 1888 SergiWinogradsky devised a selective enrichment technique for phototrophic bacteria; the “Winogradsky Column”. The Winogradsky column is an example of a mixed culture method. Its purpose is to help determine and demonstrate the growth of microorganisms, while monitoring their activity over a period of time. • So what? Why is important? Is it important?

6. Before you leave • Pick up the trash around you • Place your chairs on the tables • Have a good day! • Remember your homework.