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Objective: SWBAT understand how DNA technology influenced the area of Forensics

Using this picture as a guide, explain how gel electrophoresis works. Objective: SWBAT understand how DNA technology influenced the area of Forensics. Quiz on Monday Notebook Checks on Thursdays. DNA fingerprinting.

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Objective: SWBAT understand how DNA technology influenced the area of Forensics

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  1. Using this picture as a guide, explain how gel electrophoresis works. Objective: SWBAT understand how DNA technology influenced the area of Forensics

  2. Quiz on Monday • Notebook Checks on Thursdays

  3. DNA fingerprinting • Because DNA is as unique to you as your fingerprints, the term used to analyze human DNA is called DNA fingerprinting Four main steps • Extracting DNA • Cutting DNA • Sorting segments • Analyzing the results

  4. DNA fingerprinting • Because DNA is as unique to you as your fingerprints, the term used to analyze human DNA is called DNA fingerprinting Four main steps • Extracting DNA • Cutting DNA • Sorting segments • Analyzing the results

  5. DNA fingerprinting • Because DNA is as unique to you as your fingerprints, the term used to analyze human DNA is called DNA fingerprinting Four main steps • Extracting DNA • Cutting DNA • Sorting segments • Analyzing the results

  6. Isolation of DNA • Break down membranes to reach DNA • Break down proteins to release DNA • Separate DNA from mixture Once the DNA is isolated, it is able to be characterized

  7. 2. Cutting the DNA • The whole DNA molecule is too complex to characterize • Characterize pieces of fragments of DNA

  8. Restriction Enzymes- found in bacteria used to cut DNA in smaller fragments A restriction enzyme will recognize a specific sequence of bases and cut the DNA molecule at a specific point

  9. For example • Restriction Enzyme - • Cuts between G and C T TT G C C C A A AA C G G G T TTTG CCCA AAAC GGGT 1 2

  10. For example • Restriction Enzyme - EcoRi • Finds the sequence GAATTC • Cuts between G and A G A A T T C C T T A A G G A T TT G A T T C C G A T T C C T A AA C T A A G G C T A A G

  11. For example • Restriction Enzyme - EcoRi • Finds the sequence GAATTC • Cuts between G and A G A A T T C C T T A A G G A T TT G A T T C C G A T T C C T A AA C T A A G G C T A A G

  12. For example • Restriction Enzyme - EcoRi • Finds the sequence GAATTC • Cuts between G and A G A A T T C C T T A A G G A T TT G A T T C C G A T T C C T A AA C T A A G G C T A A G 1 2 3

  13. RFLP • R- Restriction enzymes are used to cut DNA into • F- fragments that are many different • L- lengths and exhibits • P- polymorphism (‘many shapes’)

  14. Simulation of RFLP Purpose In this activity you will take a long strand of simulated DNA and use simulated restriction enzymes to cut the strand and make a DNA fingerprint. Using a comparison of fragments lengths, you will then analyze the DNA fingerprints to determine the perpetrator of a crime

  15. Simulation of RFLP Purpose In this activity you will take a long strand of simulated DNA and use simulated restriction enzymes to cut the strand and make a DNA fingerprint. Using a comparison of fragments lengths, you will then analyze the DNA fingerprints to determine the perpetrator of a crime

  16. Post-lab Questions Answer in complete sentences and turn in on separate sheet of paper • SUMMARIZE THE PURPOSE OF THIS LAB INTO YOUR OWN WORDS • WHICH SUSPECT IS THE PERPORTRATOR? • WHY ARE RESTRICTION ENZYMES USED FOR DNA FINGERPRINTING? • IS CUTTING BETWEEN THE A AND G ON THE TOP SEQUENCE THE SAME AS CUTTING BETWEEN THE G AND A ON THE BOTTOM SEQUENCE? EXPLAIN OR ILLUSTRATE • DESCRIBE THE STEPS USED BY SCIENTIST TO MAKE A DNA FINGERPRINT. • SHOW HOW THE FOLLOWING DNA SEQUENCES WOULD BE CUT BY THE RESTRICTION ENZYME Hae III Hae 111 identifies the sequence GGCC and cuts between the G and C GGCCATGGCCATG CCGGTACCGGTAC

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