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(b)

( a ). (b). (c). 94 DCRD 97. p65wt. 19. 551. ATG.  NH 2 p65. pCMV-  NH 2 p65 -tag. pCMV-  NH 2 p65 -tag. pCMV- p65 D94E;D97E -tag. pCMV- p65 D94E;D97E -tag. pCMV-p65wt-tag. pCMV-p65wt-tag. 97. pCMV-p65wt. pCMV-p65wt. 75. 75. p65wt-tag. p65wt-tag. p65wt.  NH 2 p65-tag.

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(b)

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  1. (a) (b) (c) 94DCRD97 p65wt 19 551 ATG NH2p65 pCMV-NH2p65-tag pCMV-NH2p65-tag pCMV-p65 D94E;D97E-tag pCMV-p65 D94E;D97E-tag pCMV-p65wt-tag pCMV-p65wt-tag 97 pCMV-p65wt pCMV-p65wt 75 75 p65wt-tag p65wt-tag p65wt NH2p65-tag NH2p65-tag 50 50 IB: āp65 COOH IB: āFLAG Cytosol Cytosol (d) pCMV-p65 D94E;D97E-tag pCMV-p65wt-tag pCMV- NH2p65-tag 75 p65wt-tag IP: āFLAG IB: āp65 COOH, āp50 and āIB p65wt NH2p65-tag 50 p50/NFB1 IB 37 Cytosol

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