70 likes | 235 Views
High-Throughput Analysis of Genomic Data [S6] E NRIQUE B LANCO. NUMBER OF SLIDES: 5 EXPECTED TIME: 15 minutes. ChIPseq BIOINFORMATICS ANALYSIS. Quality Control. Peak Calling. Mapping. Visualization. QUALITY CONTROL. PROGRAM: FASTQC FORMATS: FASTQ QSEQ.
E N D
High-Throughput Analysis of Genomic Data [S6]ENRIQUE BLANCO NUMBER OF SLIDES: 5 EXPECTED TIME: 15 minutes
ChIPseq BIOINFORMATICS ANALYSIS Quality Control Peak Calling Mapping Visualization High-Throughput Analysis of Genomic Data [S6] - ENRIQUE BLANCO 2013 eblanco@ub.edu
QUALITY CONTROL • PROGRAM: • FASTQC • FORMATS: • FASTQ • QSEQ http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ High-Throughput Analysis of Genomic Data [S6] - ENRIQUE BLANCO 2013 eblanco@ub.edu
MAPPING • PROGRAMS: • BOWTIE • GEM • ELAND • FORMATS: • ELAND • SAM Genome-wide identification of DNA-protein interactions using chromatin immunoprecipitation coupled with flow cell sequencing. Hoffman BG and Jones SJ. J Endocrinol. 2009 High-Throughput Analysis of Genomic Data [S6] - ENRIQUE BLANCO 2013 eblanco@ub.edu
VISUALIZATION • PROGRAMS: • UCSC • GBROWSE • FORMATS: • WIG • BEDGRAPH S. Perez-Lluch, E. Blanco, A. Carbonell, D. Raha, M. Snyder, F. Serras and M. Corominas. Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing. Nucleic Acids Research 39:4628-4639. High-Throughput Analysis of Genomic Data [S6] - ENRIQUE BLANCO 2013 eblanco@ub.edu
PEAK CALLING • PROGRAMS: • PEAKSEQ • MACS • FORMATS: • BED • GFF S. Perez-Lluch, E. Blanco, A. Carbonell, D. Raha, M. Snyder, F. Serras and M. Corominas. Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing. Nucleic Acids Research 39:4628-4639. High-Throughput Analysis of Genomic Data [S6] - ENRIQUE BLANCO 2013 eblanco@ub.edu
High-Throughput Analysis of Genomic Data [S6]ENRIQUE BLANCO NUMBER OF SLIDES: 5 EXPECTED TIME: 15 minutes