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The 5 I’s of Culturing Microbes

The 5 I’s of Culturing Microbes. I noculation: introduction of sample into a container of media I ncubation: under conditions that allow growth I solation: separating 1 species from another I nspection I dentification. Media Provides Nutrients for Bacteria.

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The 5 I’s of Culturing Microbes

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  1. The 5 I’s of Culturing Microbes • Inoculation: introduction of sample into a container of media • Incubation: under conditions that allow growth • Isolation: separating 1 species from another • Inspection • Identification

  2. Media Provides Nutrients for Bacteria • Nutrient broth: liquid media (trypticase soy broth, TSB) • Nutrient agar: solid media (trypticase soy agar, TSA) • Agar slants (tubes) • Agar plates • Agar: polysaccharide isolated from red algae • Solid at room temp (25oC) • Liquid at 100oC • Provides framework to hold moisture & nutrients

  3. Inoculation

  4. Incubation & Isolation

  5. Pure vs Mixed Cultures • Eschericia coli (white) • Micrococcus luteus (yellow) • Serratia marcescens (red)

  6. Isolation Technique

  7. Fig. 3.3

  8. Inspection & Identification

  9. Bacteria • 1) Bacillus = Rod shaped. (pl. bacilli) • (diplobacilli, streptobacilli) • 2) Coccus = Round shaped. (pl. cocci) • (diplococci, streptococci, staphylococci) • 3)Spiral = Spiral shaped • (spirilla, vibrio, spirochete)

  10. Bacteria may appear as single cells or in groups: • These terms describe typical bacteria groupings: • 1) diplo = paired cells • 2) strepto = long chains • 3) staphylo = grape-like clusters

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