1 / 14

What is the single most important item in your home?

Cotton Candy Capillaries H. Ron Greene II, Biomedical Engineering, University of Rhode Island BME 281 First Presentation, October 4, 2011 <hrgreene2@my.uri.edu>. What is the single most important item in your home?. The fridge? The TV? The Wii?. Indoor Plumbing!.

brygid
Download Presentation

What is the single most important item in your home?

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cotton Candy Capillaries H. Ron Greene II, Biomedical Engineering, University of Rhode Island BME 281 First Presentation, October 4, 2011 <hrgreene2@my.uri.edu>

  2. What is the single most important item in your home? The fridge? The TV? The Wii?

  3. Indoor Plumbing! Without Indoor plumbing , modern society would be set back 250 years.

  4. Engineered Tissues Need Toilets Too Until recently, tissue engineers have been unable to provide plumbing for fabricated tissues. Urethra grown in vitro This has greatly hindered advancement in terms of creating larger and more complex tissues and organs. ↑ This is real people! ↑

  5. There is no <CTRL> + C on Mother Nature’s plumbing (or MAC) Mammalian cells utilize capillary networks for the delivery of nutrients and the removal of waste Capillary networks are arranged so that the cells which they nourish are located within 100 – 200 µm

  6. Major Fail Absence of vascular network has limited thickness of clinically used engineered tissues to mm scale at most Failure to provide nutrient supply frequently results in loss of more than 95% of transplanted cells

  7. How did those guys come up with this!? Leon Bellan Dr. Jason Spector After giving a university lecture Dr. Jason Spector was approached by a student working with microfibers Leon Bellan noted that the dimensions and general form of cotton candy mimics capillaries

  8. They made cotton candy, and the rest was history Vascular microchannels were made with store bought sugar and a modified cotton candy machine

  9. Do you have a recipe for that? Step 1: Pour molten sugar to form thin stems. Let cool. Step 2: Make cotton candy. Step 3: Stick sugar stems into cotton candy. Step 4: Incubate for 2 minutes to fuse sugar structure. Step 5: Submerge in biocompatible epoxy. Let cure. Step 6: Use ethanol to dissolve sugar. Rum should do. Step 7: Fill with blood. Enjoy.

  10. Will this actually work? electron microscopy Frames from video of flourescent polystyrene particle flow. multiphoton spectroscopy

  11. Take a closer look at this Red blood cells in vitro Red blood cells in epoxy

  12. And there ya have it ladies and gentlemen Final Product: physiologically functional microvascular network in biocompatible PDMS cast.

  13. Captain’s Log: Stardate 2000 and somethin’ The ability to construct microvascular networks is a major hurdle passed on the road to engineering complex organs.

  14. Phat Shout Out To: References [1] URI BME 281 BME Seminar II <www.ele.uri.edu/courses/bme281>. [2] Ikada, Yoshito. Tissue Engineering: Fundamentals and Applications.Kidlington, Oxfordshire: Elsevier Inc, 2006, pgs 63-64 [3] Leon M. Bellan, Sunil P. Singh, Peter W. Henderson, Teresa J. Porri, Harold G. Craighead, Jason A. Spector. "Fabrication of an artificial 3-dimensional vascular network using sacrificial sugar structures." Soft Matter, 2009: pgs 1354-1357. [4] Jason W. Nichol, Ali Khademhosseini. "Modular tissue engineering: engineering biological tissue from the bottom up." Soft Matter, 2009: pgs 1312-1319. [5] “Making Capillaries from Cotton Candy.” Dean of Invention, Planet Green, 2011. Television

More Related