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Announcements

Announcements. Exam 3 is Wednesday, 3/28 Material for Exam 3: Lectures 19-25 Key terms #7-9 (key terms 9 are from cancer lecture; will be posted later today) Homeworks 6-7 (NOT homework 5) Same format as previous exam. BIO 304 Lecture 26. Lac repressor bound to DNA.

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Announcements

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  1. Announcements • Exam 3 is Wednesday, 3/28 • Material for Exam 3: • Lectures 19-25 • Key terms #7-9 (key terms 9 are from cancer lecture; will be posted later today) • Homeworks 6-7 (NOT homework 5) • Same format as previous exam

  2. BIO 304 Lecture 26 Lac repressor bound to DNA

  3. Regulation of gene expression

  4. Some terminology Inducible enzyme: Constitutive enzyme: Repressible enzyme: Positive gene regulation: Negative gene regulation: Cis-acting site: Trans-acting factor:

  5. The lac operon

  6. The lac operon

  7. The lac operon

  8. In the lac operon model, which of these components is a cis-acting element? 1. The lac repressor ✓ 2. The lac operator 3. The beta-galactosidase enzyme 4. Lactose

  9. Genetic proof of the operon model

  10. For the following E. Coli genotypes, determine what the phenotypes would be in the presence of lactose: inducible, permanently repressed, or constitutively active? 1. I+ O+ Z+ 2. I- O+ Z+ 1. inducible; inducible 2. repressed; constitutive ✓ 3. inducible; constitutive 4. repressed; inducible 5. constitutive; repressed

  11. Positive control of the lac operon

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