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Emulsification. To Demonstrate the emulsification of neutral fats in water and solutions of Sodium Carbonate, Soap and Bile salt. Emulsion. Emulsion is a colloidal dispersion in which both the disperse phase and dispersion medium are liquids but are not miscible. Principle.
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Emulsification To Demonstrate the emulsification of neutral fats in water and solutions of Sodium Carbonate, Soap and Bile salt.
Emulsion • Emulsion is a colloidal dispersion in which both the disperse phase and dispersion medium are liquids but are not miscible.
Principle • When Oil and water which are not miscible, are shaken together , the oil is broken up into very tiny droplets which are dispersed in water. This is known as oil in water emulsion. The water molecules due to their high surface tension have a tendency to come together and form a separate layer. That's why the oil and water emulsion is unstable.
In the presence of substances that lower the surface tension of water for example, Sodium carbonate, soap, Bile salt etc. The tendency of water molecules to coalesce is decreased and emulsion become stable. Since , bile salt cause the greatest decrease in surface tension , they are the best emulsifying agents.
Reagents • 1. Sodium Carbonate Solution. • 2. Soap Solution. • 3. Bile Salt Solution.
Procedure • Take 5ml each of water, Sodium carbonate solution, soap solution and bile salt solution in separate test tubes and label them as A, B, C, D. • Add 3-5 drops of oil in each test tube. • Shake well and allow it to stand for 10 minutes.
Interpretation • Oil and water separate quickly indicating that the emulsion is unstable. • In case of Sodium Carbonate separation occurs after some time , indicating that emulsion is more stable than water. • In Soap Solution separation takes still longer indicating that the emulsion is fairly stable. • In case of bile salt solution there is no separation, indicating that emulsion is most stable.