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(1000) (26) (137) (110) (56) (73)

Mutations transcriptional activity (%) dox-sensitivity. - - F86Y V9I F86Y F67S F86Y F86Y G138D V9I F86Y G138D F86Y V9I F86Y F67S F86Y F86Y G138D V9I F86Y G138D F86Y V9I F86Y F67S F86Y F86Y G138D V9I F86Y G138D. 100

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(1000) (26) (137) (110) (56) (73)

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  1. Mutations transcriptional activity (%) dox-sensitivity - - F86Y V9I F86Y F67S F86Y F86Y G138D V9I F86Y G138D F86Y V9I F86Y F67S F86Y F86Y G138D V9I F86Y G138D F86Y V9I F86Y F67S F86Y F86Y G138D V9I F86Y G138D 100 3878 1001 1017 1849 1435 1707 337 346 685 764 796 1300 1653 3047 2336 2892 (1000) (26) (137) (110) (56) (73) (68) (390) (276) (144) (219) (129) (138) (113) (16) (41) (22) sc-rtTA rtTA N-terminal TetR domain C-terminal TetR domain Both TetR domains 10 1000 100000 0 40 80

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