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32 P

32 P. phospho (Ser) PKC Ab. Autoradiography. Rbt475. + Ca 2+ (50 µM). - Ca 2+. IP 3 R1. IP 3 R3. IC 50 ≤ 2 µM. Ca 2+. mass spectrometry: IP 3 R1 and IP 3 R3 performed by Prof. E. Waelkens (Biochemie). Phosphorylated receptor → SDS-PAGE In gel digestion

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32 P

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  1. 32P phospho (Ser) PKC Ab

  2. Autoradiography Rbt475

  3. + Ca2+ (50 µM) - Ca2+ IP3R1 IP3R3 IC50 ≤ 2 µM Ca2+

  4. mass spectrometry: IP3R1 and IP3R3 performed by Prof. E. Waelkens (Biochemie) • Phosphorylated receptor → SDS-PAGE • In gel digestion • Extraction of proteolytic peptides • Concentration of phosphopeptides • Mass spectrometry

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