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NMR Experimental Parameters and Applications: Chemical Shifts, Linewidth, Signal Decay Times, Splittings, and More

Discover the various experimental parameters that can be measured by NMR, including chemical shifts, linewidth, signal decay times, and splittings. Explore the applications of NMR in dynamics, structure determination, binding studies, metabolic analysis, and more.

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NMR Experimental Parameters and Applications: Chemical Shifts, Linewidth, Signal Decay Times, Splittings, and More

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  1. NMR part II • Experimental parameters that can be measured by NMR: - chemical shifts- linewidth- signal decay times- splittings Biophysical Tools '04 - NMR part II

  2. Precession Larmor frequency wL = -g B0 Biophysical Tools '04 - NMR part II

  3. Larmor Frequency Biophysical Tools '04 - NMR part II

  4. Free Induction Decay Biophysical Tools '04 - NMR part II

  5. Fourier Transform of FID Biophysical Tools '04 - NMR part II

  6. Applications: Dynamics - T1 relaxation Biophysical Tools '04 - NMR part II

  7. Applications: Structure - J-coupling Biophysical Tools '04 - NMR part II

  8. Applications: Binding - Chemical exchange Slow exchange: k << Dw Fast exchange: k >> Dw 1/T2obs = 1/T2 + k • Applications: • Rotational rates • On-, off rates • Ligand binding Biophysical Tools '04 - NMR part II

  9. Intracellular pH Biophysical Tools '04 - NMR part II

  10. Metabolism Glucose metabolism Energetics Biophysical Tools '04 - NMR part II

  11. Molecular Ordering Biophysical Tools '04 - NMR part II

  12. NMR Imaging Biophysical Tools '04 - NMR part II

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