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효소와 전기영동

효소와 전기영동. 1. Nuclease - Exonuclease - Endonuclease. - Exonuclease. ( E. coli ). ( Alteromonas espejiana ). ( Aspergillus oryzae ). - Endonuclease. ( 암소의 췌장에서 채취 ). ( 제한효소 ). Restriction enzyme Type I Type II.

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효소와 전기영동

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  1. 효소와 전기영동

  2. 1. Nuclease - Exonuclease - Endonuclease

  3. - Exonuclease (E. coli) (Alteromonas espejiana)

  4. (Aspergillus oryzae) - Endonuclease (암소의 췌장에서 채취) (제한효소)

  5. Restriction enzyme • Type I Type II

  6. 2. Ligase

  7. 3. Polymerase 1. The need for high fidelity in copying the base sequence. 2. DNA polymerase can only function in on direction. 3. The need for speed (the replication fork moves at 1000 nucleotides per second in E. coli.)

  8. 4. Modifying enzyme

  9. 5. Topoisomerase Topoisomerase I Two classes of antibiotics that inhibit gyrase are the coumarins (including novobiocin), and the quinolones (including nalidixic acid and ciprofloxacin).

  10. 제한효소의 발견과 기능

  11. 제한효소의 기능

  12. Blunt end and Sticky end

  13. Restriction enzyme site in DNA Tetranucleotide (GATC) = 44 = 256 Hexaanucleotide (GGATCC) = 46 = 4096 λ DNA (49kb) = Bgl II 6개 BamH I = 5개 Sal I = 2개

  14. Restriction enzyme의 활용 1unit – 50ul의 volume에서 λ DNA 1ug을 1시간 동안 완전히 자르거나 붙일 수 있는 양

  15. Determining the % agarose to use for separating DNA fragments.1 1Taken from Qiagen News, 1999. 2 The best resolution will be at the bottom of the range.

  16. Reagent Recipes

  17. Loading buffer recipes Optimal Voltage and Electrophoretic Times

  18. Dye Mobility Table: Migration of double-stranded DNA in relation to Bromophenol Blue (BB) and Xylene Cyanol (XC) in SeaKem LE Agarose Gels (in base pairs).

  19. 아가로스젤 (Agarose gel) 에서의 DNA 확인

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