Life Analytical Chemistry-Molecular Imaging (MI): Nuclear Imaging

1. Life Analytical Chemistry-Molecular Imaging (MI): Nuclear Imaging Gaolin Liang （梁高林）, Ph. D. Professor, Ph. D. Advisor Deptartment of Chemistry University of Science and Technology of China

2. “异域秋分暑未休 平居四载有乡愁 人生岂尽如心事 更忆霜白叶落秋” By Wei Huang, Ph.D. candidate Stanford University

3. “孤身疾影下庐阳 报国为家意欲畅 舒拳展腿犹未达 纷花乱眼仍断肠” By Gaolin Liang, Professor USTC

4. PET Fusion techniques: PET/CT Nuclear Imaging SPECT Fusion techniques: SPECT/CT Practical concerns in nuclear imaging The amount of radiation from diagnostic nuclear medicine procedures is kept within a safe limit and follows the "ALARA" (As Low As Reasonably Achievable) principle.

5. PET的名字来源于： Positron，正电子， Emission，发射， Tomography，断层摄影术。

7. Using18F,11C,15O, 64Cu…for PET imaging Oxygen (15O, T1/2 = 2 min); nitrogen (13N, T1/2 = 10 min); carbon (11C, T1/2 = 20 min); fluorine (18F, T1/2 = 110 min), copper (64Cu, T1/2 = 12 h) and iodine (124I, T1/2 = 4 days).

8. [18F]-fluoro-deoxy-glucose

9. Physiologic distribution of [18F]-FDG After i.v. injection, [18F]-FDG is distributed in the body like glucose. In accord with the glucose consumption of the cells, it is taken up by the glucose transport proteins, mainly by the GLUT-I, and phosphorylyzed by the hexokinase. Unlike glucose, [18F]-FDG does not take part in further steps of the glucose metabolism, which leads to a trapping of the molecule in the cells. Due to increased anaerobic glycolysis, many tumor cells show an up regulation of GLUT-I and of hexokinase, which together with physiologic trapping leads to an accumulation of FDG. In normal tissues, [18F]-FDG does not show a uniform distribution. The most intense physiologic uptake is seen in brain tissue, which is diagnostically used for a wide range of indications in neurology and psychiatry. [18F]-FDG is mainly excreted by the kidneys.

10. [18F]-FDG- HEAD AND NECK

11. [18F]-FDG- MYOCARDIUM

12. [18F]-FDG- GASTROINTESTINAL TRACT

13. [18F]-FDG- GENITOURINARY

14. [18F]-FDG- MUSCULAR ACTIVITY

15. [18F]-FDG- THYMUS UPTAKE

16. [18F]-FDG- BONE MARROW

17. [18F]-FDG- SPLENIC UPTAKE

18. [18F]-FDG- BENIGN PATHOLOGIC CAUSES OF 18F-FDG UPTAKE

19. Probes, applications, and instrumentations

21. 18F-SFB Synthesis

22. Procedure • 1 mL K222/K2CO3 (15 mg/ml & 3 mg/ml in 9:1 ACN/H2O) • drying • 4-5 mg precursor, 1 ml anhydrous DMSO, 120 °C for 20 min • Add 10 mL H2O, C18 cartridge, wash once with 5 mL H2O • 3 mL ACN (with 50 μL Bu4NOH) to rinse the cartridge • 20 mg TSTU, 90 °C for 10 min • HPLC purify, Rt: 23.4-24.4 min (7-37min: 5 – 65 % ACN) • C18 cartridge, wash it once (5 mL H2O) • 1 mL ACN rinse the cartridge (18F-SFB) • Typical peptide labeling (Lys side chain): 10 mCi 18F-SFB + 0.4 mg peptide Total time: 1 h Decay-corrected yield: 30-60% (5-10 peptides) • Typical protein labeling (Lys side chain): 10 mCi 18F-SFB + 0.4 mg protein Total time: 30-40 min Decay-corrected yield: 1-30% (3-5 proteins)

23. Liang, G.L.; et al. Unpublished results.

24. Xiaoyuan (Shawn) Chen, Ph.D. National Institute of Biomedical Imaging and Bioengineering (NIBIB)

25. Single Photon Emission Computed Tomography (SPECT)

26. Wikipedia

27. Structures of Tc-99m probes BZM Sestamibi MDP ECD

28. SPECT/CT Qingqing Miao, Xiaoyu Bai, Yingying Shen, Bin Mei, Jinhao Gao, Li Li, and Gaolin Liang*. Intracellular self-assembly of nanoparticles for enhancing cell uptake. Chemical Communications. 2012, 48 (78), 9738-9740.

29. SPECT/CT

30. SPECT/CT FOR SLN MAPPING

31. SPECT/CT IN SKELETAL DISEASES- Applications in Malignant Skeletal Diseases

32. SPECT/CT IN DIFFERENTIATED THYROID CANCER

33. SPECT/CT IN PARATHYROID TUMORS

34. SPECT/CT IN TUMORS OF SYMPATHETIC NERVOUS SYSTEM AND ADRENOCORTICAL TUMORS

35. Thanks for your attention!