1 / 17

Response of human mesenchymal stem cells to modified biomaterial surfaces

Response of human mesenchymal stem cells to modified biomaterial surfaces. Mura McCafferty Young Persons’ World Lecture Competition Kuala Lumpur September 2010. Tissue Engineering. Holds the promise of being able to produce functional tissue and organs for transplant. Skin. Trachea.

carmelita
Download Presentation

Response of human mesenchymal stem cells to modified biomaterial surfaces

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Response of human mesenchymal stem cells to modified biomaterial surfaces Mura McCafferty Young Persons’ World Lecture Competition Kuala Lumpur September 2010

  2. Tissue Engineering Holds the promise of being able to produce functional tissue and organs for transplant Skin Trachea Bladder Ear

  3. Stem Cells

  4. Mesenchymal Stem Cells (MSCs) • Can be sourced from the bone marrow, peripheral blood, adipose tissue, dental pulp and synovium • Can be easily isolated and expanded in culture to generate large numbers of cells • Multipotent - capable of producing cells of different lineages Magnification 40X

  5. Mesenchymal Stem Cells

  6. Bone Tissue Engineering • Bone struggles to heal large defects caused by: • Disease • Non-union fracture • Age • Tumors • Congential Deformations • Commonly used methods to aid healing of large defects include: • Intramedullary pins • Plating • Synamic external fixation • Bone grafts

  7. MSCs and bone tissue engineering • Dexamethasone • L-ascorbic acid-2-phosphatase • β-glycerophosphate • Expensive • May cause adverse reaction to body Expand MSCs in vitro Isolate MSCs from patient Induce osteogenic differentiation Transplant cells back to defect site

  8. MSCs and bone tissue engineering • There is therefore an existing need to develop alternative methods of inducing the osteogenic differentiation of MSCs • One such route: FUNCTIONAL BIOMATERIALS

  9. Control of osteogenic differentiation through modified biomaterials • The chemical and physical properties of a biomaterial has a significant effect on cell behaviour • It is thought, and has been demonstrated in some studies, that surface topographical features have the potential to induce osteogenic differentiation of MSCs • Dalby, M.J., et al., The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat Mater, 2007. 6(12): p. 997-1003. • Sjostrom, T., et al., Fabrication of pillar-like titania nanostructures on titanium and their interactions with human skeletal stem cells. Acta Biomater, 2009. 5(5): p. 1433-41. • Bigi, A., et al., In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy. J Biomed Mater Res A, 2007. 82(1): p. 213-21. MSCs cultured on planar PMMA MSCs cultured on embossed PMMA

  10. Sputter Deposition Previous published studies have shown that sputter deposited titanium (Ti) and calcium phosphate (CaP) thin films can promote bone cell attachment and proliferation Sputter deposition is a thin film coating process that has been used successfully to provide bioactive layers that are inherently osteoconductive Sputter deposited Ti and CaP thin films have promising potential for directing the osteogenic differentiation of MSCs 500 nm CaP Titanium Substrate

  11. Substrates Glass Ti Ti/CaP AFM images showing 3D analysis of substrates

  12. MSCs cultured in osteogenic media Alizarin Red Staining DAY 14 DAY 28 DAY 21 DAY 7 NORMAL MEDIA Magnification 40x OSTEOGENIC MEDIA Magnification 40x

  13. Gene expression analysis - Collagen I mRNA expression of Collagen I in MSCs cultured on control, Ti and Ti/CaP substrates

  14. Gene expression analysis - Osteopontin mRNA expression of Osteopontin in MSCs cultured on control, Ti and Ti/CaP substrates

  15. Immunocytochemical Localisation of Osteopontin Control (Normal Media) Control (Osteogenic Media) Ti Ti/CaP

  16. Summary While MSCs hold great promise for use in bone tissue engineering applications, there is a need for more effective in vitro expansion and differentiation methods • Reduce dependence on bone graft treatment • Improve healing • Reduce recovery time CaP and Ti sputter deposited surfaces have utility in directing the osteogenic differentiation of MSCs While these are promising results and further research to fully understand if this is as a result of the surface topography as supplied by the Ti coating or by the potential bioactive nature of the CaP

  17. Acknowledgements • Professor Brian Meenan • Dr George Burke • Dr Peter O’Hare • Linzi Charters • Chris O’Kane • Dr Fiona McKavanagh

More Related