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Relevant Ingredients for Algal Cultures

Explore the relevance of nickel in nutrient solutions for algal cultures, comparing growth rates with varying concentrations to optimize productivity. Workshop insights and experimental data provided by Frederic Mößbe Moldaenke from FH Kiel.

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Relevant Ingredients for Algal Cultures

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  1. Relevant Ingredients for Algal Cultures Frederic Mößbbe Moldaenke / FH KielBiomonitoring Workshop 201031st May, 2010

  2. previous ingredients experiments an additional ingredient Development of a new nutrient solution

  3. PreviousIngredients ingredients were...: • CaCl2*2H2O (Calcium chloride dihydrate) • CO(NH2)2 (Urea) • K2HPO4 (Dipotassium phosphate) • KH2PO4 (Monopotassium phosphate) • MgSO4*7H2O (Heptahydrate) • “Woods Hole” • problem between algae and nutrient solution 5: • previous nutrient solution was unstable

  4. Experiments theory: nickel is missing from nutrient solution 5 • comparison between: • nutrient solution 5, • nutrient solution 5 with 0.4 µg nickel concentration • acidic/alkaline solution

  5. Experiments Test set-up • measurements: • volume of fermenter in l • algae concentration in µg/l

  6. Experiments • calculation of growth rate r: • growth rate r in µg*l/h • volume flow in l/h • algae concentration c in µg/l • volume of fermenter V in l

  7. Experiments • results: • nutrient solution 5 with nickel is similar to acidic/alkaline solution

  8. Experiments to discover the correct nickel concentration • comparison between: • nutrient solution 5 with 0.4 µg nickel concentration, • nutrient solution 5 with 0.8 µg nickel concentration, • nutrient solution 5 with 1.2 µg nickel concentration

  9. Experiments • result: • nutrient solution with 0.8 µg and 1.2 µg nickel are almost equal

  10. Additional Ingredient ingredients are now: • 116.0 mg CaCl2*2H2O (Calcium chloride dihydrate) • 5,000 mg CO(NH2) 2 (Urea) • 2,400 mg K2HPO4 (Dipotassium phosphate) • 1,533 mg KH2PO4 (Monopotassium phosphate) • 2,000 mg MgSO4*7H2O (Heptahydrate) • 10.0 ml “Woods Hole” • 1 µg Nickel

  11. Many thanks for your attention

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