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我们的 一米阳光

我们的 一米阳光. 一米阳光 （ 5425 、 3325 ）. T helper Drift. 主讲人：单晶莉. Case study. Objective. To investigate the effects of steroids therapy on TH1/ TH2 balance in childhood primary nephrotic syndrome(PNS). Case study. Methods.

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我们的 一米阳光

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  1. 我们的一米阳光

  2. 一米阳光 （5425、3325） T helper Drift 主讲人：单晶莉

  3. Case study • Objective To investigate the effects of steroids therapy on TH1/ TH2 balance in childhood primary nephrotic syndrome(PNS) .

  4. Case study • Methods Determination of T helper TH1 and TH2 cells percentage in 21 ongoing cases of PNS without prednisone therapy , 15 relapse cases of PNS with prednisone therapy and 9 remission cases of PNS with prednisone therapy by three color flow cytometry following whole blood cells culture , compared with 10 healthy children.

  5. Case study • Results The percentages of TH1 and TH2 cells in healthy children were (13. 42 ± 4. 36) % and (2. 53 ±1. 97) % respectively. In the patients with ongoing PNS , the percentages of TH1 and TH2 cells were significantly decreased [ (2. 34 ±2. 09) % and (1. 02 ±0. 96) % respectively ] . In the patients with relapse PNS , the percentage of TH1 cells was also significantly decreased [ (1. 20 ±0. 91) % ] , but the percentage of TH2 cells was significantly increased [ (7. 65 ±4. 53) % ] , and the decrease of TH1 cells was more obvious than in onset. In the remission patients with PNS , the TH1 and TH2 cells percentages were normal [ (12. 04 ±4. 92) % and (2. 81 ±2. 65) % respectively ] .

  6. Case study • Results On the other hand , TH1/ TH2 ratio was 5. 41 ±2. 77 in healthy children , normal in the remission patients with PNS(4. 25 ±2. 12) , low in the patients with PNS in onset (2. 43 ±2. 65) and lower in relapse cases than in onset (0. 77 ±1. 07) .

  7. Case study • Conclusion The beneficial effects of steroids therapy can be explained by the shift from the TH2 cells to TH1 cells in children with primary nephrotic syndrome , which is primarily caused by the relatively predominant TH2 cells in vs TH1 cells.

  8. The balance of Th1 ＆ Th2 The balance of Th1 ＆ Th2 is Distroyed

  9. Topic Normally, Th1/Th2 is a balance system. 1 The balance shift to Th1 or Th2 is called T helper drift. 2 It is associated with many diseases. 3

  10. The balance of Th1 ＆ Th2

  11. The balance of Th1 ＆ Th2 Introduction of Th cells

  12. What is Th cell look like?

  13. Introduction of Th Cells Classification of Th1/Th2 cells 1 Differentiation of T helper cells 2 Biological functions of Th1/Th2 3

  14. 1、Classification of Th1/Th2 cells Cytokines they secret 1986 Mosmann mouse 1991 Romagnani human Th1 Th2 • According to the present/absent of CD4+/CD8+ Cytotoxic T lymphocytes (CTL) CD8+ T helper cells (Th) CD4+

  15. 2、 Differentiation of T helper cells • Clone conversion克隆转化: when CD4+ cell is in the premise appearance (Th0), it only expresses IL-2. Under the function of different antigens、cytokines and APC , Th0 cell is converting to Th1 or Th2.

  16. Differentiation and functionof Th1 and Th2

  17. Usually,Th1 cells do not act on target cells directly,but kill intracelullar pathogens or allo- target cells through recuiting and activating Macrophages indirectly.So,macrophage is the principle effector cell of Th1 cells. Biological Functions of Th1 cells

  18. Biological Functions of Th1 (1)Th1 IL-8,other chemokines recruit more macrophages (2)Th1 IL-3, GM-CSF induce HSC to differentiate more macrophages (3)Th1 IFN- ,CD40L activate macrophage (4)Ag presentation more phagocytosis

  19. Biological Functions of Th1 cells • Active macrophage • Anti-intracellular pathogen infection(virus、bacteria and parasite) • Promote cytotoxic • Delayed type hypersensitivity;DTH

  20. Biological Functions of Th1

  21. Th1 Cell Help our body clear the source of infection Advantages Disadvantages Cause the harm of our body easily

  22. Biological Functions of Th1/th2 induce humoral immunity Th2 cell • Th2 cells can stimulate the immunoreponse of B cells to protein Ag by direct contact with B cells or secreting CKs IL-4 stimulate B cells proliferation and production of antibodies • IL-4、IL-5 stimulate mast cells and eosinophil Mast cell involves in hypersensitivity 1 Eosinophil involves in anti-parasites

  23. Th2 Cell Protection our body via restrict the destructiveness of Th1 Advantages Disadvantages Often cause chronic diseases

  24. S u m m a r y

  25. T helper drift and diseases Infectious diseases Hypersensitivity disease Autoimmune response Graft rejective reaction

  26. For example • The correlation of TH1/TH2 shift and gastric cancer

  27. For example The correlation of TH1/TH2 shift and gastric cancer • Pathogeny: Quantity of Th1 cells and cytokines secreted by them ↓,Quantity of Th2 cells and cytokines secreted by them ↑. Th1/Th2 ↓, All this lead to the inhibition of anti-tumor immunity.

  28. For example The correlation of TH1/TH2 shift and gastric cancer • Therapy : In patients of gastric cancer Th1 cell is inhibited, while on the contrary ,Th2 cell is activated . In this conditions, IL-12 can be used to stimulate the proliferation of Th1 cells, so as to recover the balance of Th1 /Th2

  29. Thanks for listening • 主讲人： 单晶莉 • ppt制作：胡 杰 赵 婷 • 资料搜集：韩海峰 赵 凯 贺 毅 • 技术支持：张月雷 戚庆庆

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