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The Ameliorative Effect of Nigella Sativa Oil in Lead-Intoxicated Rats

This study investigates the potential of Nigella Sativa oil to mitigate genotoxicity and oxidative damage caused by lead intoxication in rats. Clinicopathological studies were conducted to evaluate the effects of Nigella Sativa oil on genotoxicity, serum biochemical changes, oxidative stress, and histopathological alterations in the liver, kidneys, and brain.

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The Ameliorative Effect of Nigella Sativa Oil in Lead-Intoxicated Rats

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  1. بسم الله الرحمن الرحيم With The Name Of ALLAH

  2. Clinicopathologicalstudies on the ameliorative effect of Nigella sativa oil against genotoxicity and oxidative damage in lead intoxicated rats دراسات باثولوجية اكلينيكية علي التأثير الملطف لزيت حبة البركة ضد التغيرات السمية الوراثية و التلف التأكسدي في الفئران المسممة بالرصاص.

  3. Nashwa, A. Abu-Aita ; Sherein, S. A. EL Gayed; Mogda,K.Mansoutr(2006) : Clinico pathological studies on the ameliorative effect of nigella sativa oil against genotoxicity and oxidative damage in lead intoxicated rats. J. Egypt. Vet. Med. Assoc. Vol 66 No4 ; 127–46.

  4. Introduction

  5. Poisonous substances in the environment (xenobiotics) are numerous and have toxic and carcinogenic properties that cause many disease problems Leadis a common environmental and industrial pollutant that has been detected in all phases of the environment and biologic systems It belongs to the group ofmost toxic heavy metalsin the atmosphere

  6. Lead causes a wide range of toxic-biochemical effects in man and animals Liver Target organs Kidneys Brain

  7. Mechanism of action Several mechanisms have been suggested to explain how lead induce its toxic effect Oxidative stress is considered as one of the important mechanisms of toxic effects of lead. Lead causes oxidative damage through – --inducing the generation of reactive oxygen species (ROS) --reducing the antioxidant defense systems

  8. Plant antioxidant Recent investigations have shown that the antioxidant properties of plants could be correlated with oxidative stress defense Nigella sativa is a one of the native herbaceous plant belonging to family Ranunculacea It is widely distributed in Egypt and other Arabian countries.

  9. Aim of the work

  10. The present investigation was carried out to Evaluate the effect of Nigella sativa oil

  11. Genotoxicity Biochemical Serum changes Oxidative stress Histopathological alterations

  12. Materials and Methods

  13. Experimental design

  14. Sampling

  15. 1-Bone marrow samples Collected from both femurs For micronucleus test.

  16. 2- Serum samples Serum biochemical analysis ALT,AST,ALP Choles.,TP.,ALB.,GLOB BUN,CRT Phosphorus,Calcium

  17. 3- Tissuesspecimens Liver Kidneys Brain Oxidative stress biomarkers Histopathological alterations TBARS GHS CAT

  18. Results

  19. Genotoxicity Micronucleus test

  20. Effect of Nigella sativa oil on the frequency of MPCE in bone marrow cells of lead intoxicated rats.

  21. Biochemical results

  22. Serum biochemical analysis

  23. Oxidative stress biomarkers

  24. Histopathological alterations

  25. Liver of rats gr.3(lead acetate treated group), showing several hepatic changes in the form of; pleomorphism ,binucleation, vacuolation ,and karyolysis. (H&E X200).

  26. Liver of rats gr.4(pre-treated group of lead with N.sativa), showing thrombus in the blood vessel consisting of fibrin network with trapped leucocytes.(H&EX200).

  27. Liver of rats gr.5(concurrent treated group with lead and N.sativa), showing apparently normal hepatic tissue.(H&EX200).

  28. Liver of rats gr.6(post-treated group of lead with N.sativa), showing focal area of coagulative necrosis infiltrated with mononuclear cells.(H&EX200).

  29. Kidnys of rats gr.3(lead acetate treated group), Showing complete absense of the glomerular tufts and vacuolation of the cytoplasm of most tubular epithelium. (H&EX200).

  30. Kidnys of rats gr.3(lead acetate treated group), Showing cytomegaly and karyomegaly of proximal tubular cells.(H&EX400).

  31. Kidnys of rats gr.4(pre-treated group of lead with N.sativa), Showing Vacuolation of the glomerular tufts and the cytoplasm of most tubular epithelia, together with congestion in both peritubular and glomerular capillaries.(H&EX200).

  32. Kidnys of rats gr.5(concurrent treated group with lead and N.sativa), Showing slight edema under the bowman capsule ,with slight congestion.(H&EX200).

  33. Kidnys of rats gr.6(post-treated group of lead with N.sativa), Showing dilatation in the glomerular space,and shrinked glomerular tufts.(H&EX200).

  34. cerebrum of rats gr.3(lead acetate treated group), Showing degenerated • neurons, satellitosis, and neuronophagia.(H&EX200).

  35. cerebrum of rats gr.4&6(pre&post-treated groups of lead with N.sativa), Showing diffuse perivascular and cellular edema.(H&EX200).

  36. cerebrum of rats gr.5(concurrent treated group with lead and N.sativa), Showing focal area of hemorrhage ,with mild cellular edema.(H&EX200).

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