0.02

1. 0 12 24 36 48 60 Time (h) 0.04 0.03 H2O (0 h) Relative bioluminiscence 10 μM Cu (3 h) 0.02 10 μM Cu (6 h) 0.01 0 0 0 12 12 24 24 36 36 48 48 60 60 72 72 Time (h) Time (h) 0.06 0.04 Relative bioluminiscence BCS Cu 0.02 0 A 0.04 0.03 MS Relative bioluminiscence 0.02 Cu 0.01 0 B C C Figure S6.Copper effects on the luciferase activity driven by the LHY promoter in Arabidopsis seedlings. A, Bioluminescence from the PLHY::luc seedlings grown on MS plates either without (MS, white circles) or with 10 μM CuSO4 (Cu, black circles), entrained under 7 neutral days with photocycle and thermocycle and transferred to continuous light. Bioluminiscence was measured every hour after transferring to continuous light. Values are the seedling means ± SD (n ≥ 21). The light and dark bars below the panel indicate subjective day and night, respectively. B, Bioluminescence from the PLHY:luc seedlings grown on MS plates entrained under 7 neutral days with photocycle and thermocycle and transferred to continuous light, and then subjected to the addition of H2O after 0 h (white circles), 10 μM CuSO4 after 3 h (gray circles) or 10 μM CuSO4 after 6 h (black circles). Bioluminiscence was measured every hour after transferred to continuous light. Values are the seedlings average (n = 4-6). Arrows indicate the addition time. C, Bioluminescence of the PLHY:luc seedlings grown on MS plates with 10 μM CuSO4 for 6 days and grown on MS with 10 μM CuSO4 (Cu, black circles) or 100 μM BCS (BCS, white circles) for 1 additional day, under neutral days with photocycle and thermocycle and transferred to continuous light.Bioluminiscence was measured every hour after transferring to continuous light. Values are the seedlings average (n = 4).