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Advanced Polarization Toolbox for Fluorescence Studies

The Electro-Optical L-Format (EOLF) module allows easy measurement of polarization, anisotropy, and circularly polarized luminescence. Traditional methods updated with EOLF for precise gain correction and rapid polarization state switching. Ideal for kinetic experiments, without a gain correction factor and needing no polarizer movement. Supports CPL and excitation anisotropy spectra with advanced properties. Enhance your research with EOLF's complementary techniques on a single optical bench.

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Advanced Polarization Toolbox for Fluorescence Studies

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  1. Polarization Toolbox Electro-Optical L-Format (EOLF) Polarization of Fluorescence Module Add Anisotropy and Circularly Polarized Luminescence Easily

  2. Traditional L-Format Anisotropy

  3. IHV G = Gain Correction: IHH IV V – GIVH P = IV V + GIVH Polarization: IV V – GIVH r = Anisotropy: IV V + 2GIVH Traditional Calculation of Anisotropy and Polarization

  4. Anisotropy with New EOLF

  5. IV V – IVH Polarization: P = IV V + IVH IV V – IVH r = Anisotropy: IV V + 2IVH Calculation of Anisotropy and Polarization using an EOLF Module

  6. EOLF Advantages • No Gain Correction Factor (G-Factor) • No polarizer movement • Rapid switching between polarization states • supports high speed kinetic experiments

  7. EOLF Chamber, PEM version

  8. EOLF Chamber in a DM 45 Spectrofluorimeter

  9. Comparison of PEM and LCVR Properties

  10. Photon Counter Gating

  11. Polarization Anisotropy Excitation Anisotropy Spectrum of Rhodamine

  12. Fluorescein Spectra as a Function of Temperature temperature Increasing

  13. EOLF Also Supports CPL Circularly Polarized Luminescence (CPL) • Differential emission of left and right circularly polarized light • Fluorescence analog of circular dichroism (CD) • Sensitive to environmental changes of chiral fluorophores

  14. 30000 Left polarized 20000 Right polarized Intensity, counts 10000 0 570 580 600 610 620 630 590 Emission Wavelength, nm Left and Right Polarized Emission Spectra Europium(III) tris(trifluoromethylhydroxymethylene)-d-camphorate inDMSO

  15. CPL Spectrum of Europium Compound

  16. CPL with Polarized Excitation Use circular polarized excitation to photoselect one enantiomer in a racemic mixture

  17. US Patent Pending

  18. EOLF Adds Complementary Techniques to a Single Optical Bench

  19. With One EOLF, Measure: • Conformation characterization of proteins and other biological macromolecules • Equilibrium binding studies of macromolecules • Characterization of metal ligand complexes • Characterization of entantiameric mixtures

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