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Bacteria All Around Us!!! Prelab Questions. How do antibiotics kill bacteria? 4 ways!!! Why must researchers continuously search for new antibiotics? Why do antibiotics not affect human cells they way they affect bacterial cells?
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Bacteria All Around Us!!!Prelab Questions • How do antibiotics kill bacteria? • 4 ways!!! • Why must researchers continuously search for new antibiotics? • Why do antibiotics not affect human cells they way they affect bacterial cells? • Why should we be careful when we use antibiotics and not take them when we have colds or the flu?
Bacteria All Around Us!!!Exit Slip What kind of evolution are bacteria experiencing? Micro or Macro. Explain your answer
Bacteria All Around Us!!! • What is the difference between: • Antiseptics • Antibacterial • Antibiotics • Disinfectants • Can you name examples of each?
Bacteria All Around Us!!! • Antiseptics • Inhibits the growth and reproduction of disease-causing microorganisms on living tissues • Disinfectants • Destroys microorganisms on non-living objects • Antibacterial • Destroy or inhibits the growth of bacteria • Antibiotics • Medicines that destroy or inhibits bacterial cells within the body
Bacteria All Around Us!!!Hypothesis • Antiseptic-Mouthwash • Antibacterial-Softsoap, Germ-x • Antibiotics-Kanamycin/Erythromycin • Disinfectants-Liqui-nox, Bleach, Lysol • What do you think will inhibit the growth of E.coli the best? • Write your hypothesis in the form of a if-then becausestatement.
ProcedureWrite down or Draw Each Step Day One (Tomorrow) • Draw a X on the bottom of the petri dish, and label the section A-B-C-D • Swab the E.coli on the agar. Draw and S shape, turn, draw another S shape • Place a control disk in the center of section A • Place a Antibacterial soap in section B Place a Sanitizer in section C Place a Disinfectant in section D • Tape petri dish closed and write your name on it
ProcedureWrite down or Draw Each Step Day Two (Monday) 6. Do not open dish! 7. Look for clear zones around the disks. Bacteria will be growing everywhere else. Draw in your results. 8. Measure the diameters of these zones in millimeters and record in the data table.
AntibioticsGram - / Gram + E.coli is gram – negative • Kanamycin: Gram – negative, works! • Erythromycin: Gram + positive, does not work. Gram + positive Gram – negative (E.coli)