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Research-based interdisciplinary skills across the curriculum

Research-based interdisciplinary skills across the curriculum. ASM Meeting 2009 Rupa Iyer PhD Director of Biotechnology Programs Director of Center for Life Sciences Technology University of Houston. Academic Program. 4- year interdisciplinary major leading to a B. S. degree

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Research-based interdisciplinary skills across the curriculum

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  1. Research-based interdisciplinary skills across the curriculum ASM Meeting 2009 Rupa Iyer PhD Director of Biotechnology Programs Director of Center for Life Sciences Technology University of Houston

  2. Academic Program • 4- year interdisciplinary major leading to a B. S. degree • Collaboration with Industry and Academic Partners • Two tracks, Bioprocessing and Bioinformatics.

  3. Laboratory Curriculum • Designing new laboratory modules • In collaboration with Melinda Wales, Ph.D. Reactive Surfaces and Texas A & M

  4. Laboratory Curriculum • Project Based • Modular • Model for interdisciplinary programs

  5. Project - Based • Soil bacterium Pseudomonas dimutins • Plasmid encoded gene responsible for degradation of organophosphorous compounds (OP) • OP gene identified, cloned, expressed and the upstream and downstream processes of protein production characterized. • Applications in Environmental Biotechnology

  6. IRBC

  7. Module I :Environmental Sampling and Enumeration • Sample Collection • Isolation and Characterization of Isolates • Obtain soil samples and isolate OP hydrolyzing colonies on primary culture plates. • Pesticide ( paraoxon , parathion ) used as sole sources of C, S, P

  8. Module II: Remediation of Organophosphorous (OP) Compounds • Cloning of the gene encoding organophosphorous hydrolases (opd) • Plasmid Isolation • Restriction Digest • Ligation and Transformation • Enzymatic Assay of OPH

  9. Module III: Bioprocessing Good Manufacturing Practices • Cell Growth • Bioreactors • Upstream Processing • Downstream Processing • Introduction to Nanotechnology

  10. Beta Testing • Module I and II • Brigham Young Hawaii – Fall 2007 • UH - Research Experience for Undergraduates, Summer 2008 • Fall UH BTEC 3100 , Research Methods and applications

  11. Beta Testing • Module III BTEC 4398 Special topics in Bioprocessing • Students develop parameters such as temperature, pH, and aeration for optimal bacterial growth in bioreactors. • Generate data when they manipulate that specific parameter. • They will share and interpret their data to propose optimal growth conditions for their bacterial culture.

  12. Assessment and Evaluation Assessment • Research Paper • Lab Note Book • Quizzes Evaluation • Student assessment of learning gains

  13. Research Experience • Field Activity: Module I • Students collect soil samples • Test for the presence of oph activity • Location of oph activity • Analyze data • Collaborations: students test for the presence of oph (and pesticide contamination ) activity

  14. Linking Research Experiences • Electronic Lab Notebook • Student upload their own data • Compare data with one another • Submit a research paper • Based on popular open source package (Drupal)

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