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Electrochemical detection of lung cancer specific microRNAs using 3D DNA origami nanostructures

This study presents a 3D DNA origami nanosensor for highly sensitive detection of lung cancer-specific microRNAs. The sensor design, preparation process, and characterization using SEM, AFM, CV, and DPV are discussed. Under optimal conditions, the genosensor exhibited great potential in clinical cancer diagnosis applications.

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Electrochemical detection of lung cancer specific microRNAs using 3D DNA origami nanostructures

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  1. Electrochemical detection of lung cancer specific microRNAs using 3D DNA origami nanostructures Ondřej Zítka Datum konání: 20.11.2015 Investice do rozvoje vzdělávání

  2. Background • Recent reports have indicated that aberrant expression of microRNAs is highly correlated with occurrence of lung cancer. • Therefore, highly sensitive detection of lung cancer specific microRNAs provides an attractive approach in lung cancer early diagnostics.

  3. The probe for sensor • The 3D DNA origamistructureisconstitutedof a ferrocene-tagged DNA of stem-loopstructurecombinedwith a thiolatedtetrahedron DNA nanostructureatthebottom. • The top portionhybridizedwiththelungcancercorrelatedmicroRNA, whilethebottomportionwasself-assembled on gold disk electrodesurface, whichwasmodifiedwithgoldnanoparticles (Au NPs) and blockedwithmercaptoethanol (MCH).

  4. Scheme of the genosensor

  5. Characterisation of sensor • The preparation process and the performance of the proposed electrochemical genosensor were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and differential pulse voltammetry (DPV).

  6. Characterisation of sensor (A) CV curves o fgold disk electrodes before (b) and after (a) deposition of gold nanoparticles in 0.5M H2SO4 from 0 to1.5V at a scan rate of100mV/s.(B) SEM image for the AuNPs-coatedgoldelectrode. AFM images for the (C) bare,(D) AuNPs-coatedand (E) 3D DNA probe/Au NPs-coated gold electrodes.

  7. Performance of electrochemical genosenzor (A) DPV for the electrochemical genosensor in the presence of target mi-croRNAs at 1mM,100nM,10nM,1nM,100pM,10pM and 0pM (from top to bottom). (B) The linear relationship between peak current increasement and microRNA concentration. (C) DPV curves of electrochemical genosensor applied in 1 mM microRNA dissolved in RNAse-free PBS(b) and cell lysis(a).

  8. Summary • Under the optimal conditions, the developed genosensor had a detection limit of 10 pM and a good linearity with microRNA concentration ranging from 100 pM to 1 µM, which showed a great potential in highly sensitive clinical cancer diagnosis application.

  9. Project: Paper: Acknowledgements: Liu, S.P., Su, W.Q., Li, Z.L. and Ding, X.T. (2015) ElectrochemicaldetectionoflungcancerspecificmicroRNAsusing 3D DNA origaminanostructures. Biosensors & Bioelectronics, 71, 57-61. Budování výzkumných týmů a rozvoj univerzitního vzdělávání výzkumných odborníků pro mikro- a nanotechnologie (NANOTEAM) CZ.1.07/2.3/.00/09.0224 Investice do rozvoje vzdělávání

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