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Ikkitousen

Ikkitousen. Quang Truong Josh Garcia BIOL 1750.501 12/02/13. Collection Site Data. Quang Truong discovered the soil in the backyard of his home that he dug up from under his Japanese Maple Tree. The soil was moist and measured at 98 degrees Fahrenheit at 3:16 pm on July 22,2013.

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Ikkitousen

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  1. Ikkitousen Quang Truong Josh Garcia BIOL 1750.501 12/02/13

  2. Collection Site Data • Quang Truong discovered the soil in the backyard of his home that he dug up from under his Japanese Maple Tree. The soil was moistand measured at98 degrees Fahrenheitat3:16 pm on July 22,2013. • 32.609463 N • 97.042534 W

  3. Tree

  4. Overview Phage Collection/ Morphology • Size/ Measurements of Capsid – 63.713 nm • Tail - 241.658 nm • Head to Tail – 306.691 nm • Morphotype– Siphoviridae(Capsid diameter about 60 nm) Ex: Adephagia, Denton TX, 2009,

  5. Electron Microscopy (EM)

  6. Electron Microscopy (EM)

  7. DNA Yield/ Quality • Concentration of DNA: 226.5 ng/ul • Current Volume as of 11/18/13: 65.2 ul • Current Total ug of DNA as of 11/18/13: 14.7678 ug • Phage Titer of HTL: 8.0 x 10^9 pfu/ml

  8. RE/ Cluster/ Genome Size • Genome Size: N/A • Cluster: K (Due to similarities in comparison to Adephagia, Angelica, Emerson, etc.

  9. Ladder • Cut 1- 212 • Cut 2- 229.333 • Cut 3- 254.667 • Cut 4- 266.667 • Cut 5- 294.667 • Cut 6- 338.667 • Cut 7- 390.667 • Cut 8- 438.667 • Cut 9- 506.667 • Cut 10- 613.333

  10. Discussion/Comparison & Cluster ID - We narrowed down our phage to fall within Cluster K using the (PET) website. - After determining our cluster, we were able to narrow down a sub-cluster in which we determined to be K1. - Examples: Adephagia, Angelica, Emerson, & Amelie. - In comparison to the other four clusters that fall under cluster K, we found that a few of the other Restriction Digestions had cuts under “ClaI” in the K4 Sub-cluster. - Cuts of “BamHI,” & “HindIII” under K5 Sub-cluster.

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