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BIO210A Lab Instructor: E. Schmid, Ph.D. Lab Assignment Sheet I (RFLP Analysis Example A). Student Name. Date. 6,800 bp (= 100%). DNA probe. Gene. = Bgl-II restriction sites. Normal gene ( N- allele ). *. *. = Point mutation. Gene. Mutant gene ( M - allele ). DNA (bp).
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BIO210A Lab Instructor: E. Schmid, Ph.D. Lab Assignment Sheet I(RFLP Analysis Example A) Student Name Date 6,800 bp (= 100%) DNA probe Gene = Bgl-II restriction sites Normal gene(N- allele) * * = Point mutation Gene Mutant gene(M- allele) DNA(bp) RFLP pattern (after Southern Blotting) Genotype NN NM MM