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AGRP

Suplemmentary figure 1. A. KLF4. SF-1 protein levels ( arbitrary units ). **. Arcuate. Ventromedial. B. C. SF-1. -Actin. Arcuate. Lateral. AGRP. **. -Actin. Suplemmentary figure 2. A. B. Ad-GFP. Ad-KLF4. C. Energy expediture (Kcal/kg/48h).

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AGRP

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  1. Suplemmentary figure 1 A KLF4 SF-1 proteinlevels (arbitraryunits) ** Arcuate Ventromedial B C SF-1 -Actin Arcuate Lateral AGRP ** -Actin

  2. Suplemmentary figure 2 A B Ad-GFP Ad-KLF4 C Energy expediture (Kcal/kg/48h) Total locomotro activity (beam brakes/48h) RQ (vCO2/vO2) 24h RQ (vCO2/vO2) 0 5 17 24 (hours)

  3. Suplemmentary figure 3 A B Ad-GFP Ad-KLF4 ARC ARC C Daily food intake (gr) Cummulative food intake (gr) Ad-GFP 500 µm Body weight gain (gr) Daily weight gain (gr)

  4. Suplemmentary figure 4 A * Ad-GFP Ad-KLF4 Ad-GFP ACC Ad-KLF4 * pACC FAS LPL WAT protein levels (arbitrary units) * HSL pHSL 50 µm -Actin C 100 µm B 100 µm ** Ad-GFP Ad-KLF4 Ad-KLF4 Ad-GFP Adipocyte area (%) µg TG/0.1 gr liver 50 µm

  5. Suplemmentary figure 5 A Lentiv-GFP SD Lentiv-GFP HFD Lentiv shRNA-KLF4 SD Lentiv shRNA-KLF4 HFD B * C Cummulativefoodintake (gr) D

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