Week : 4 .ERT 144 staining (rev, quiz) isolation & identification

1. Week : 4 .ERT 144staining (rev, quiz) isolation & identification

2. Preparations of Specimens for Microscopes

3. safranin ---- ---- ---- Malachite green

4. Congo Red ---- ---- ----

5. Mycobacterium tuberculosis Mycobacterium Leprae 24 years old man infected with leprocy gram

6. simple stains require only a single dye and a simple uncomplicated procedure, differential stains use 2 differently colored dyes : primary dye and the counterstain, Special stains are used to emphasize certain cell parts that are not revealed by conventional staining methods • simple staining uses the ready binding of bacterial cells to dyes like malachite green, • crystal violet, basic fuchsin, and safranin. • It reveals bacterial characteristics such as shape, size, and arrangement • all cells, regardless of type, appear almost the same color • Gram staining (Hans Christian Gram ) : • Universal diagnostic staining technique for bacteria. • gram-positive, >stain purple • gram-negative, >stain pink (red). • is the basis of important bacteriological topics egtaxonomy, cell wall structure, The different results in the Gram stain are due to differences in the structure of the cell wall (peptidoglycans) and how it reacts to the series of reagents applied to the cells.

7. Gram Staining

10. tr Streak plate

12. D