1 / 16

بسم الله الرحمن الرحيم

بسم الله الرحمن الرحيم. Oxidative Decarboxylation and Krebs Cycle. By. Amr S. Moustafa, M.D.; Ph.D. Clinical Biochemistry Unit, Pathology Dept. College of Medicine, King Saud University. ALT. Alanine. PLP. αKG. Glutamate. Fates of Pyruvate. Oxidative Decarboxylation of Pyruvate.

chinara
Download Presentation

بسم الله الرحمن الرحيم

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. بسم الله الرحمن الرحيم

  2. Oxidative Decarboxylation and Krebs Cycle By Amr S. Moustafa, M.D.; Ph.D. Clinical Biochemistry Unit, Pathology Dept. College of Medicine, King Saud University

  3. ALT Alanine PLP αKG Glutamate Fates of Pyruvate

  4. Oxidative Decarboxylation of Pyruvate Allosteric Regulation

  5. PDH Complex: Covalent Regulation Insulin + PDH Insulin Glucagon - PDH P + Pi H2O Protein Phosphatase Pyruvate dehydrogenase complex (active) Pyruvate dehydrogenase complex (inactive) Protein Kinase ATP ADP + Glucagon

  6. Tricarboxylic Acid Cycle: Krebs Cycle • Final common pathway for oxidation • Exclusively in mitochondria • Major source for ATP • Mainly catabolic with some anabolic features • Synthetic reactions (anabolic features): • Glucose from amino acids • Nonessential amino acids • Fatty acids • Heme

  7. Krebs Cycle

  8. Krebs Cycle Reactions (1)

  9. Krebs Cycle Reactions (2) Succinate Thiokinase Substrate-Level Phosphorylation

  10. Glycolysis (Cytosol) Phospho-glycerate Kinase 2 2 Substrate- Level Phosphorylation 2 2 2 Substrate- Level Phosphorylation Pyruvate Kinase 2

  11. Krebs Cycle Reactions (3)

  12. Krebs Cycle: Energy Yield

  13. Krebs Cycle: Energy Yield

  14. Net ATP Production byComplete Glucose Oxidation Aerobic glycolysis: 8 ATP Oxidative decarboxylation: 2 X 3 = 6 ATP Krebs cycle: 2 X 12 = 24 ATP Net: 38 ATP

  15. Take Home Message • Pyruvate is oxidativelydecarboxylated by PDH to acetyl CoA inside the mitochondria • Krebs cycle: • Final common pathway for the oxidation of carbohydrates, fatty acids and amino acids • occurs in the mitochondria • Aerobic • Mainly catabolic, with some anabolic reactions • The complete oxidation of one glucose molecule results in a net production of 38 ATP molecules

  16. Thank you

More Related