50 likes | 157 Views
Structural and chemistry analysis of native and malted barley endosperm by polarized Raman spectroscopy (PRS). Leonardo Galvis , Carlo Bertinetto , and Tapani Vuorinen Coimbra: 8-9 May 2014. Polarized Raman Spectroscopy (PRS).
E N D
Structural and chemistry analysis of native and malted barley endosperm by polarized Raman spectroscopy (PRS) Leonardo Galvis, Carlo Bertinetto, and TapaniVuorinen Coimbra: 8-9 May 2014
Polarized Raman Spectroscopy (PRS) Vibrational spectroscopy technique for Chemical and Physical analysis of materials (0.6-1μm resol., non-destructive) Raman mean spectra of a starch granule (native barley) of an area depicted as blue square on the starch granule sketch.
Raman images of compounds present in the interface aleuronelayer-endosperm in barley seed (Multivariate analysis). non-starch carbohydrates starch protein ferulicacid native malted
Polarizationmeasurements in starchgranules A) Optical microscopy image of the endosperm in native barley. B) Raman image of the area band ratio 865cm-1/485 cm-1at 0opolarization angle of the laser. C) Ramanimage of the arearatio of bands 865 cm-1/485cm-1 at 90opolarizationangle of the laser.
Anysotropicresponseofglycosidic band (865 cm-1) B) A) 1 2 3 A) AFM image in topography mode of a sectioned starch granule with ellipsoidal shape found in native barley endosperm. (B) Experimental and fittedanysotropicresponse of the bandratio 865cm-1/485cm-1 on the areasmarked as (1), (2) and (3) respectively in the samestarchgranule.