1 / 2

以連續互補微陣列晶片分析陰道鞭毛蟲卡路里限制相關基因表達

以連續互補微陣列晶片分析陰道鞭毛蟲卡路里限制相關基因表達.

chloe-levine
Download Presentation

以連續互補微陣列晶片分析陰道鞭毛蟲卡路里限制相關基因表達

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 以連續互補微陣列晶片分析陰道鞭毛蟲卡路里限制相關基因表達以連續互補微陣列晶片分析陰道鞭毛蟲卡路里限制相關基因表達 • 陰道鞭毛蟲為WHO宣佈為最廣泛的非病毒性性接觸傳染疾病，已證實會造成HIV感染機率增加、與子宮頸癌、不孕症有關聯性，且其細胞週期的G2期較G1期長，但對陰道鞭毛蟲的仍不清楚。之前曾將陰道鞭毛蟲培養於低糖的卡路里限制液態培養基，發現會增加其存活時間，本研究想研究陰道鞭毛蟲於卡路里限制時其轉錄體基因調控機制，利用之前所建立的三個基因表達序列資料庫︰TvEST（5934條序列），TvCS（9941條序列）及TvG2M（10687條序列），進行複製擴增得到7668非重複性序列當作架構陰道鞭毛蟲微陣列晶片之探針。以卡路里限制培養，選擇延滯期、對數期及生長穩定期不同生長時期進行微陣列晶片分析，發現與卡路里限制相關的基因，明顯減少的有Hydrogenosomal malic enzyme (AP65-1)、Cyclophilin type 5、hypothetical proteins及MAPKKK，明顯增加的有28S ribosomal RNA protein, 40S ribosomal protein S3Ae, zzz-NO HIT與不同的hypothetical proteins，再以RT-PCR加以證實微陣列晶片之實驗結果。推論在卡路里限制時陰道鞭毛蟲之DNA複製、protein turnover相關基因是表達量增加的；蛋白質合成、醣解作用、產生能量相關基因是表達量降低的。

  2. Serial analysis of calorie restriction-associated gene expression in Trichomonas vaginalis by cDNA microarray • Trichomonas vaginalis is one of the most common non-viral sexually transmitted disease, and trichomoniasis is emerging as one of the most important cofactors in amplifying HIV transmission, also linked to this disease are cervical cancer and infertility. However, very little is known about the biology of T. vaginalis at molecular level. We aimed to study the effect of calorie-restriction on the growth of this unicellular parasite. Previous studies showed that calorie restriction can increase the life-span of many model organisms. A T. vaginalis cDNA microarray (TvARRAY V1) was fabricated by using 7668 nonredundant expressed sequence tags (EST) selected from three cDNA libraries: TvEST(5934 sequences), TvCS(9941 sequences) and TvG2M(10687 sequences).At lag、logarith- mic and stationary phases under calorie-restriction comparative micro- array analysis reveals calorie-restriction related genes including, downre- gulation：Hydrogenosomal malic enzyme (AP65-1),cyclophilin type 5, MAPKKK and Hypothetical proteins；upregulation：28S ribosomal RNA protein, 40S Ribosomal protein S3Ae, zzz-NO HIT and hypothetical pro- teins. Reverse transcription PCR analysis of selected genes also showed similar results, thereby supporting the reliability of the TvARRAY system. Thereby,calorie-restriction produced changes in gene expression consistent with increased DNA replication and protein turnover related genes；which decreased protein synthesis、glycolysis and energy production related genes.

More Related