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Physical insulation of pathways or functions

Physical insulation of pathways or functions. How to build complex synthetic circuits?. Electronic hard wired: reusability of components. Biology is diffusive: crosstalk We need to find a way to insulate the different components circuits. Pathway insulation methods.

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Physical insulation of pathways or functions

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  1. Physical insulation of pathways or functions

  2. How to build complex synthetic circuits? • Electronic hard wired: reusability of components. • Biology is diffusive: crosstalk We need to find a way to insulate the different components circuits.

  3. Pathway insulation methods Orthogonality (chemical insulation) Family of orthogonal parts Physical insulation Scaffolding Synthetic organelles Synthetic ecosystems

  4. PROJECT 2:BUILDING A NEW ORGANELLEFOR SYNTHETIC BIOLOGY A quick recap wild-type cell create “synthesome” use “synthesome” STEP 2: Recruit proteins to carryout any synbio process of choice (e.g. drug or biofuel factory) STEP 1: Create spatially distinct membrane compartment that has unique molecular identity

  5. An external organelle Targetting Components of a pathway to secretion External organelles producing cell External organelles producing cell

  6. An external organelle External organelle Chassis (Feeder)

  7. An external organelle Attachment transmembrane proteins Chassis (Feeder)

  8. External organelle Chassis (Feeder)

  9. External organelle Chassis (Feeder) communication

  10. External organelle composition (1) Function? Receiver factory Chassis (Feeder) Chassis (Feeder) sender

  11. Advantage: part reusability Example: multicolor Hasty oscillator State output Chassis (Feeder) Chassis (Feeder) output

  12. External organelle composition (2) pathway product Chassis (Feeder) Starting product

  13. Natural example: Nanoarchaeum equitans

  14. Nanoarchaeum: ABC transporters Chassis (Feeder) Communication: Within eukaryotes, ABC-transporters mainly transport molecules to the outside of the plasma membrane or into membrane-bound organelles such as the endoplasmic reticulum, mitochondria, etc.

  15. Natural examples Gap junctions Bacterial conjugation

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