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Northern blotting

Northern blotting. Electrophoresis. We can separate DNA and RNA molecules by size using agarose gel electrophoresis. DNA (or RNA) samples loaded into wells. Ethidium bromide is fluorescent in UV light. Northern, Southern, Western. In the 1970s Ed Southern of Oxford University invented a

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Northern blotting

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  1. Northern blotting

  2. Electrophoresis • We can separate DNA and RNA molecules by size using agarose gel electrophoresis

  3. DNA (or RNA) samples loaded into wells

  4. Ethidium bromide is fluorescent in UV light

  5. Northern, Southern, Western In the 1970s Ed Southern of Oxford University invented a revolutionary DNA blotting technique. The Southern Blot allows the visualization of one DNA fragment from a whole genome DNA extract. Ed Southern: Possibly regrets this photo

  6. Northern and Western • People then applied the same technique to RNA. • They called it a “Northern blot”. Funny, eh? • Then other people applied it to protein, and imaginatively called it a “Western blot”

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