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Supplementary Figure 4. A. B. Ctl sip300. Ctl siPCAF. p300. PCAF. ERCC1. ERCC1. Actin. Actin. Cisp (2h). Cisp (2h).
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Supplementary Figure 4 A B Ctl sip300 Ctl siPCAF p300 PCAF ERCC1 ERCC1 Actin Actin Cisp (2h) Cisp (2h) PCAF and p300 are not involved in ERCC1 accumulation in response to cisplatin. H358 cells were transfected for 72 hours with either control (Ctl), p300 (A) or PCAF (B) siRNA and exposed to 50M cisplatin for an additional 2 hours. Western blotting was performed using anti-PCAF, anti-p300 and anti-ERCC1 antibodies. Actin was used as a loading control