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CI-710 Miniature Spectrometer

CI-710 Miniature Spectrometer. Tuesday, October 22, 2013 Conducted by: Brienne Meyer bmeyer@cid-inc.com. Agenda/Outline. CI-710 hardware CI-710 Theory of Operation SpectraSnap! Software Competing Instruments. Selling Points. Three points to make Accuracy Portability Ease of Use.

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CI-710 Miniature Spectrometer

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  1. CI-710 Miniature Spectrometer Tuesday, October 22, 2013 Conducted by: Brienne Meyer bmeyer@cid-inc.com

  2. Agenda/Outline • CI-710 hardware • CI-710 Theory of Operation • SpectraSnap! Software • Competing Instruments

  3. Selling Points Three points to make • Accuracy • Portability • Ease of Use

  4. CI-710 Miniature Leaf Spectrometer • Truly portable • Field-ready with leaf probe • Wide-range spectrum • 400nm-950nm (PAR & NIR) • High-resolution (~1.5nm) • Real-time display • Lightweight (~290g)

  5. What does the CI-710 measure? • Transmission/absorbance mode: • Light passing through the leaf • Reflectance mode: • light reflecting from leaf surface Source: www.ceramicx.com/infrared-heat/

  6. CI-710 Theory of Operation • Bifurcating fiber optic cable • Range 400-950nm • Reference light beam from 2 sources • Blue LED & Incandescent lamp • Output in the visible-to- infrared range

  7. CI-710 Theory Continued • Most photosynthetically active green leaves: • strongly absorb blue/blue-green and red light • reflect green and yellow light Source: scienceprojectideasforkids.com Source: www.shortcourses.com

  8. CI-710 Theory III • Light source has 2 positions: • transmission/absorbance or reflectance mode Transmission and Absorbance Mode Reflectance Mode • Light travels through fiber optic to leaf probe and leaf

  9. CI-710 Theory IV • Disperses light by a diffraction grating • Projects the dispersed light onto a CCD array • Each pixel corresponds to a specific wavelength of light • SpectraSnap! displays light intensity of each pixel Source: www.horiba.com

  10. CI-710 Leaf Clip • Also referred to as leaf probe • Isolates stray light • Secures specimen • In transmission or absorbance mode, • the leaf clip scatters • the source beam

  11. CI-710 Applications • Measures light transmission, absorption and reflection of leaves • Measure optical properties of glass • WengJH. et al. RELATIONSHIPS BETWEEN PHOTOSYSTEM II EFFICIENCY AND PHOTOCHEMICAL REFLECTANCE INDEX UNDER DIFFERENT LEVELS OF ILLUMINATION: COMPARISON AMONG SPECIES GROWN AT HIGH-AND LOW ELEVATIONS THROUGH DIFFERENT SEASONS. Trees-Structure and Function, volume 26, issue 2, pages 343-351, April 2012 • National Chung Hsing University, Taiwan

  12. CI-710 Applications Continued • Qualitative spectral data from interaction of light with leaf • Pigments, organic compounds, and inorganic chemicals exhibit distinct wavelength characteristics Common Plant Pigments • Leaf surface characterization • Photochemical reactions • Index of refraction Source: www.cameratechnica.com

  13. CI-710 General Points • USB powered by tablet or laptop PC: • SpectraSnap! • Waveform and wavelength values • Peaks • Pre-loaded indices SpectraSnap!

  14. SpectraSnap! • Driver installation built-into software • Analyze multiple peaks • Integrate across wavelength bands • Compare specimens • Add layers • Import previously saved layers

  15. SpectraSnap! Indices • Normalized Difference Vegetation Index (NDVI) • Plant Senescence Reflectance Index (PRSI) • Pigments: Anthocyanin, Carotenoids • Add custom algorithms/indices • Simultaneously monitor and record indices Source: www.exelisvis.com

  16. CI-710 Tips • Warm-up time: 15-30 minutes • Recalibrate often • Use reflectance standard to calibrate in reflectance mode • white circle faces down • Use a black backing on other side of leaf in reflectance mode

  17. How to Demonstrate the CI-710 • Warm up CI-710 • Connect to PC, light turned on • Calibrate • Open leaf clip • Position leaf • View data in SpectraSnap! • Add calculations • Export to spreadsheet

  18. SpectraSnap! Data

  19. Competing Instruments Source: www.stellarnet.us Source: www.asdi.com

  20. Conclusion • Fast, reliable, non-destructive measurements • How to demonstrate a leaf spectroscopy measurement • SpectraSnap! software • Free & easy to use • Wide spectrum (400-950nm)

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