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Part 1: Biological databases

Essential Bioinformatics and Biocomputing ( LSM2104: Section I) Biological Databases and Bioinformatics Software Prof. Chen Yu Zong Tel: 6874-6877 Email: csccyz@nus.edu.sg http://xin.cz3.nus.edu.sg Room 07-24, level 7, SOC1, NUS January 2003.

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Part 1: Biological databases

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  1. Essential Bioinformatics and Biocomputing (LSM2104: Section I) Biological Databases andBioinformatics SoftwareProf. Chen Yu ZongTel: 6874-6877Email: csccyz@nus.edu.sghttp://xin.cz3.nus.edu.sgRoom 07-24, level 7, SOC1, NUSJanuary 2003

  2. Essential Bioinformatics and Biocomputing (LSM2104: Section I) Four lecturesPart 1: Biological databases:Lecture 2. Biological information and databasesLecture 3. More databases, retrieval systems, and database searching Part 2: Software:Lecture 4. Examples of the applications of bioinformatics software and basic principlesLecture 5. Overview of bioinformatics software

  3. Part 1: Biological databases Part 1 outline: • Biological information and databases • Overview and definition, types of biological databases 2. Popular databases, records, data format • Genbank, SwissProt, OMIM, PDB, KEGG, BIND, Pfam, PROSITE, PubMed 3. Accessing biological databases, retrieval systems • Entrez, SRS 4. Searching biological databases • Data quality, coverage, redundancy, errors Textbook: --T.K.Atwood and D.J. Parry Smith, Introduction to Bioinformatics. Biological databases: chapters 3 and 4 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  4. Biological Information Cancer as an example: Genes: Growth Genes Tumor suppressor genes Proteins: Growth Factors Enzymes Receptors Pathways: Cell death Systems: Immune system Blood supply Function: Role of proteins Molecular interactions Essential Bioinformatics and Biocomputing (LSM2104), NUS

  5. Biological Information Nucleic acids: • DNA sequence, genes, gene products (proteins), mutation, gene coding, distribution patterns, motifs • Genomics: genome, gene structure and expression, genetic map, genetic disorder • RNA sequence, secondary structure, 3D structure, interactions Proteins: • Protein sequence, corresponding gene, secondary structure, 3D structure, function, motifs, homology, interactions • Proteomics: expression profile, proteins in disease processes etc. • Ligands and drugs (inhibitors, activators, substrates, metabolites) Essential Bioinformatics and Biocomputing (LSM2104), NUS

  6. Biological Information Pathways: • Molecular networks, biological chain events, regulation, feedback, kinetic data Function: • Binding sites, interactions, molecular action (binding, chemical reaction, etc.) • Biological effect (signaling, transport, feedback, regulation, modification, etc.) • Functional relationship, protein families, motifs, and homologs Essential Bioinformatics and Biocomputing (LSM2104), NUS

  7. Biological databases Purpose • To disseminate biological data and information • To provide biological data in computer-readable form • To allow analysis of biological data A database needs to have at minimum a specific tool for searching and data extraction. • Web pages, books, journal articles, tables, text files, and spreadsheet files cannot be considered as databases • Reading materials: • Baxevanis AD.The Molecular Biology Database Collection: 2002 update. Nucleic Acids Res. 2002 Jan 1;30(1):1-12. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  8. Biological databases Lists of biological databases • INFOBIOGEN Catalog of Databases http://www.infobiogen.fr/services/dbcat/ • Nucleic Acids Research Database Listing http://nar.oupjournals.org/cgi/content/full/30/1/1/DC1 • These serve as starting point of biological databases. • More than 500 databases have been catalogued to date and those from the two listings satisfy minimal criteria for the content, access, and quality. • Other sites as a starting point. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  9. Biological databases • INFOBIOGEN Catalog of Databases Type of database No of records DNA 87 RNA 29 Protein 94 Genomic 58 Mapping 29 Protein structure 18 Literature 43 Miscellaneous 153 Total 511 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  10. Biological databases- in Nucleic Acids Research Type of database No of records Major Sequence Repositories 7 Comparative Genomics 7 Gene Expression 20 Gene Identification and Structure 30 Genetic and Physical Maps 10 Genomic Databases 48 Intermolecular Interactions 5 Metabolic Pathways and Cellular Regulation 12 Mutation Databases 33 Pathology 8 Protein Databases 50 Protein Sequence Motifs 18 Proteome Resources 7 RNA Sequences 26 Retrieval Systems and Database Structure 3 Structure 32 Transgenics 2 Varied Biomedical Content 18 TOTAL 336 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  11. Literature databases – PubMed (MedLine) 1. It contains entries for more than 11 million abstracts of scientific publications. 2. It enables user to do keyword searches, provides links to a selection of full articles, and has text mining capabilities, e.g. provides links to related articles, and GenBank entries, among others. 3. Efficient searching PubMed requires some skill. For example, searching with a keyword “interleukin” returns 108,366 matches. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  12. PubMed web-site(http://www3.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed ) Essential Bioinformatics and Biocomputing (LSM2104), NUS

  13. PubMed Search(http://www3.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed ) Cancer treatment by targeting blood supply: Cancer growth depends on blood supply (why?) and thus requires the growth of new blood vessels – angiogenesis Proteins involved in angiogenesis may be potential anticancer targets You can find some of these targets by searching Pubmed Key word “cancer angiogenesis enzyme drug” produces 856 entries Essential Bioinformatics and Biocomputing (LSM2104), NUS

  14. Nucleic Acids databases What info are in these databases: • DNA sequence, genes, gene products (proteins), mutation, gene coding, distribution patterns, motifs • Genomics: genome, gene structure and expression, genetic map, genetic disorder • RNA sequence, secondary structure, 3D structure, interactions Essential Bioinformatics and Biocomputing (LSM2104), NUS

  15. Nucleic Acids databases DNA databases – GenBank, EMBL, DDBJ 1. General purpose databases focusing on DNA sequences and their properties 2. GenBank, EMBL-bank and DDBJ exchange data to ensure comprehensive worldwide coverage and accession numbers are managed consistently between the three centers. Reading materials: • Textbook, chapter 4 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  16. DNA databases • GenBank database(http://www.ncbi.nih.gov/Genbank/) • Contains publicly available DNA sequences from more than 100,000 organisms. • Also contains derived protein sequences, and annotations describing biological, structural, and other relevant features. • Accessible through Entrez, NCBI’s integrated retrieval system (studied later) • Sequence similarity search tools: BLAST (studied later) • EMBL nucleotide sequence database (http://www.ebi.ac.uk/embl/) • Contains nucleotide sequences collected from all public sources. • Accessible through Sequence Retrieval System (SRS) which allows keyword searching (studied later) • Sequence similarity search tools: Blitz, Fasta, and BLAST (studied later) Essential Bioinformatics and Biocomputing (LSM2104), NUS

  17. DNA databases: GenBankWeb page Essential Bioinformatics and Biocomputing (LSM2104), NUS

  18. DNA databases • An Example from GenBank– flat file • Human Alpha-Lactalbumin gene This protein is a complex of 2 proteins A and B. In the absence of the B protein, the enzyme catalyzes the transfer of galactose from UDP-galactose to Nacetylglucosamine (cf. EC 2.4.1.90). Essential Bioinformatics and Biocomputing (LSM2104), NUS

  19. A GenBank entry – HEADER Essential Bioinformatics and Biocomputing (LSM2104), NUS

  20. GenBank Entry – Links provided in the Header • MapViewer – find the gene position in chromosome • Related Sequences – other entries related to this gene (or sequence) • OMIM– link to catalog of human genes and genetic disorders • Protein – retrieve protein record from GenPept • Medline and PubMed –literature abstracts related to this gene • Taxonomy – Classification of organisms • UniGene – Unifiedgene data • UniSTS – Unifiedsequence tagged sites, marker and mapping data • LinkOut – links to publishers, aggregators libraries, biological databases, sequence centers, and other Web resources • REFSEQ – reference sequence standards Note:These links are representative. Other links may also be found in GenBank entries. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  21. GenBank entry - FEATURES Essential Bioinformatics and Biocomputing (LSM2104), NUS

  22. GenBank Entry– Links provided in the Feature section LocusID – locus and display of genomic and mRNA sequences MIM – Link to OMIM description, other entries for this sequence EC_number – link to the corresponding cataloged enzymes Protein_id – retrieve protein record from GenPept CD– conserved protein domain (SMART), CDD – conserved protein domain (Pfam). Essential Bioinformatics and Biocomputing (LSM2104), NUS

  23. Biological databases: GenBank - SEQUENCE Essential Bioinformatics and Biocomputing (LSM2104), NUS

  24. GenBank - NOTES Majority of GenBank entries have similar form to our example. When accessing the database, the following needs to be noticed: • Some entries are huge, containing as much as 30,000 lines.(NT_021877 Homo sapiens chromosome 1 working draft sequence segment) • Some entries have contig information instead of sequence information.(NT_021877 Homo sapiens chromosome 1 working draft sequence segment) • Some entries are derived from cDNA sequences and thus represent putative genes/proteins.These should be used with caution. (AK007430. Mus musculus 10 d...[gi:12840976]). • Some annotations are predicted using automated analysis. These should also be used with caution. (XM_131483 Mus musculus simi...[gi:20832685]). Essential Bioinformatics and Biocomputing (LSM2104), NUS

  25. GenBank - Statistics Year Base Pairs Sequences • 680338 606 • 101008486 78608 • 11101066288 10106023 • 15849921438 14976310 Data size is large and increases fast Essential Bioinformatics and Biocomputing (LSM2104), NUS

  26. Biological Databases Database Searching • Databases must have methods for accessing and extracting data stored. • The most basic search is keyword searching Keywords can be any word that occurs somewhere in the database records. It can be the name of the gene or protein (e.g. lactalbumin), species (e.g.homo sapiens, human), a taxonomy term (e.g.primates), or a word from the reference title (e.g. cancer) • Others include: Entry Id number, sequence • Databases typically have hyperlinks that provide access to additional information related to the entry from other sources. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  27. Biological databases: OMIM Online Mendelian Inheritance in Man (http://www.ncbi.nlm.nih.gov/Omim/) • The OMIM database contains abstracts and texts describing genetic disorders to support genomics efforts and clinical genetics. It provides gene maps, and known disorder maps in tabular listing formats. Contains keyword search. Hamosh A. et al. Online Mendelian Inheritance in Man (OMIM), a knowledge base of human genes and genetic disorders Nucleic Acids Res. 2002 30: 52-55. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  28. Biological databases: OMIM web-page Essential Bioinformatics and Biocomputing (LSM2104), NUS

  29. Biological databases: OMIM search engine Essential Bioinformatics and Biocomputing (LSM2104), NUS

  30. Biological databases: OMIM statistics All Entries : 14088 Established Gene Locus : 10476 Phenotype Descriptions : 1194 Other Entries : 2418 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  31. Biological databases Protein databases • SWISS-PROT (http://us.expasy.org/sprot/sprot-top.html) is a curated database focusing on high level of annotation (sequence, function, structure, post-translational modifications, variants, etc.) of proteins. • TrEMBL is Computer-annotated supplement to SWISS-PROT Reading materials: Textbook, chapter 3 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  32. Protein databases What are in these databases: • Protein sequence, corresponding gene, secondary structure, 3D structure, function, motifs, homology, interactions • Proteomics: expression profile, proteins in disease processes etc. • Ligands and drugs (inhibitors, activators, substrates, metabolites) Essential Bioinformatics and Biocomputing (LSM2104), NUS

  33. Protein databases– SWISS-PROT Notes: • SWISS-PROT provides high-quality annotations and detailed info about sequence, structural, functional, and other properties of proteins. • It provides a rich set of links to other sources of information on SWISS-PROT entries. Unfortunately, some of the links will not work at all times, because of the dynamical change of the Web. • It also provides a rich set of protein analysis tools. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  34. SWISS-PROT web-page Essential Bioinformatics and Biocomputing (LSM2104), NUS

  35. SWISS-PROT entry P00709 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  36. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  37. SWISS-PROT entry P00709 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  38. SWISS-PROT entry P00709 Essential Bioinformatics and Biocomputing (LSM2104), NUS

  39. Biological databases: Protein structure database: PDB (http://www.pdb.org) • More than 18,000 macromolecular structures on proteins, peptides, viruses, protein/nucleic acids complexes, nucleic acids, and carbohydrates. • Among the oldest databases – the first structure was deposited in 1972. • New deposited structures has been steadily growing (3298 in 2001, and 1486 Jan 1-June 5, 2002). • Determined mainly by the X-ray diffraction and NMR. • It Contains tools for keyword search, comprehensive visualization, and information extraction – such as sequence, geometry, and structural neighbors details. Essential Bioinformatics and Biocomputing (LSM2104), NUS

  40. Biological databases: PDB web-pagehttp://www.rcsb.org/pdb/ Essential Bioinformatics and Biocomputing (LSM2104), NUS

  41. Biological databases: A PDB entryhttp://www.rcsb.org/pdb/ Essential Bioinformatics and Biocomputing (LSM2104), NUS

  42. Biological databasesPDB statistics Essential Bioinformatics and Biocomputing (LSM2104), NUS

  43. Biological databases Summary of Today’s lecture • Types of Biological information, data and databases • Simple data retrieval method. • Popular databases: Pubmed, Genbank, SwissProt, OMIM, PDB • Statistics: • Large number of publications (MEDLINE: >12M since 1960) • Large amount of data for sequence (DNA: >14M, Protein: > 120K) • Fair amount of data for 3D structure (Protein >14K, Nucleic acid >1K) Essential Bioinformatics and Biocomputing (LSM2104), NUS

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