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Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO

Biological chemistry. Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO. Crystal engineering. Biomarker identification. Protein crystallography. Molecules. H 2. ethane. sucrose. Molecules. Molecules. Crystal. Crystal. ”Crystal engineering”, we make a supermolecule!.

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Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO

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  1. Biological chemistry Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO

  2. Crystal engineering Biomarker identification Protein crystallography

  3. Molecules H2 ethane sucrose

  4. Molecules

  5. Molecules

  6. Crystal

  7. Crystal ”Crystal engineering”, we make a supermolecule!

  8. Building blocks linker node

  9. Xe-atoms on Ni-surface (Don Eigler, 1989) Problem 1 Molecules are very small, hard to put together one by one

  10. Problem 2 As they are so small, we must put together very many before we reach a macroscopic size A 1 x 1 x 1 mm sugar crystal contains 1,4 · 1018 molecules

  11. Building blocks

  12. Building blocks

  13. Glue

  14. Intermolecular forces Must be: • strong • Directional Two main types used in CE: • Hydrogen bonds • Metal coordination

  15. MOFs • “Metal-Organic Frameworks (MOFs) are crystalline compounds consisting of metal ions or clusters coordinated to often rigid organic molecules to form one-, two-, or three-dimensional structures that can be porous”(from Wikipedia) MOF-5

  16. Can we do this without metal ions?

  17. Guanidinium derivatives Fumaric acid complex

  18. Guanidinium derivatives

  19. DNA-based systems

  20. DNA-based systems

  21. DNA-based systems

  22. Applications Construction of • polar materials for non-linear optics • molecular magnets • porous materials for storage • molecular sieves • sensors • molecule traps • biological model systems • catalysts • and much more

  23. Biomarker identification Biomarker: A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a condition or disease. A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule Present in small amounts = hard to identify

  24. Biomarker identification Nano-HPLC-system

  25. Group Lianglin Lise-Lotte Carl Henrik Malgorzata Steven Fassil Ola

  26. Protein crystallography

  27. Structure/Function Studies of Medically Relevant Systems Department of Chemistry University of Oslo Ute Krengel

  28. Methods • X-ray crystallography • Complementary techniques (molecular biology, protein chemistry, ligand binding studies, cell biology, molecular docking, organic chemistry)

  29. Targets • Glycobiological Targets • Mucins • Bacterial Toxins • Bacterial Adhesins • Anti-tumor Antibodies • Mushroom Lectins • Enzymes • Chorismate mutases GlycoNor ProtStruct

  30. Masters Projects • Glycobiological Targets: • Bacterial Toxins – Structure and Function • Investigation of blood group dependence • Delivery mechanisms? • Drug design • Anti-tumor Antibodies for Immunotherapy • Recombinant production, crystallization and X-ray structure determination

  31. Group Ute Øyvind Dani Hedda Dipankar Daniel Julie

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