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Measurement of Succinate Dehydrogenase Enzymatic Activity

Measurement of Succinate Dehydrogenase Enzymatic Activity. The Michaelis-Menten Model. Organization. One ice bucket per group of four When your filtered cauliflower juice is ready, bring your tube to the TA who is organizing the centrifugations. . Equipment notes.

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Measurement of Succinate Dehydrogenase Enzymatic Activity

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  1. Measurement of Succinate Dehydrogenase Enzymatic Activity The Michaelis-Menten Model

  2. Organization • One ice bucket per group of four • When your filtered cauliflower juice is ready, bring your tube to the TA who is organizing the centrifugations.

  3. Equipment notes • Do not write on the spec cuvettes or the polypropylene centrifuge tubes. • Do not throw away the centrifuge tubes. • Rinse all glassware and put all items (except spec cuvettes) in the dishpans. Return spec cuvettes to rack.

  4. Safety • Be careful with the sodium azide! • Cut your own parafilm and cover tubes to mix. • Place tips used to measure azide and leftover stock azide in bag on front desk • Pour reaction mixtures in labeled beaker in hood.

  5. Reminders • BEFORE YOU LEAVE: calculate initial velocity (slope) for tubes 3 and 4 and record on data sheet.

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