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Selection of BACs (IV) Assembly of BAC ends on P250I21

Selection of BACs (IV) Assembly of BAC ends on P250I21. Selection of BACs (V) Repetitive BAC end sequences on P308F14. Perfect Blast score. High quality base discrepancy Repetitive. AFLP fingerprints from chromosome 6 tomato BACs. Binning and high resolution physical map Mi Contig.

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Selection of BACs (IV) Assembly of BAC ends on P250I21

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  1. Selection of BACs (IV) Assembly of BAC ends on P250I21

  2. Selection of BACs (V) Repetitive BAC end sequences on P308F14 Perfect Blast score • High quality base discrepancy • Repetitive

  3. AFLP fingerprints from chromosome 6 tomato BACs

  4. Binning and high resolution physical map Mi Contig 3,9 kb overlap

  5. Results: • 18 Seed BACs screened against 220.000 BAC ends • On average 7 overlapping BAC-ends per seed BAC • All ‘seeds’ have candidates from 2 libraries • M008H07 overlaps 3.6kb with P125P18 • M027B07 overlaps 880bp with P153O03 • M015K08 overlaps 5.0kb with P057J04 • P103N18 overlaps 2.1kb with P046G10 • P073H07 overlaps 3.9kb with P250I21 • BlastN-analysis has been fully automated

  6. Conclusions • Proof of concept for the “BAC-end strategy” • Works for both fully closed BACs as for Phase 1 – 2 BACs • No need to fingerprint an entire BAC library • Blast, Assembly and AFLP work complementary • Accurate selection of BACs having minimal overlap and largest extending insert

  7. Taco Jesse (Poster) Kim Jansen Dennis Woltinge Jan van Haarst Thamara Hesselink Marjo van Staveren Marleen Henkens Paul Mooijman Sander Peters (Poster) René Klein Lankhorst

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