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Mshsp23

Supplementary Figure 1. A. B. Mw. P. Nt. 1. 2. 3. 4. 5. 6. 7. 8. 9. Mshsp23. C.

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Mshsp23

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  1. Supplementary Figure 1 A B Mw P Nt 1 2 3 4 5 6 7 8 9 Mshsp23 C Supplementary Figure 1. Molecular analysis of transgenic and non-transgenic tobacco plants.A Schematic representation of the T-DNA region of pCAM-MsHsp23, the expression vector used for transformation. RB, right border; LB, left border; 35S, CaMV 35S promoter; HPT, hygromycin phosphotransferase; MsHsp23, alfalfa small heat shock protein, T35S, CaMV 35S terminator; TNOS,nopaline synthase terminator; EcoRI , KpnI, XbaI and HindIIIrestriction sites. B Integration of the transgene to the transgenic plants. a PCR amplification of a 630-bp fragment of Mshsp23. C, represents the Southern blot analysis of Mshsp23 gene in transgenic tobacco. Nt non-transgenic plants

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