1 / 6

Advanced Techniques in Linear Dichroism Spectrometry for Molecular Orientation Studies

Explore polarization modulation with photoelastic modulator (PEM) for Circular Dichroism (CD) analysis. Utilize Linear Dichroism - Reflection (IRRAS) with UV or IR spectrometer and variable angle of incidence. Study peptides and proteins in membranes using Langmuir-trough setup. Discover the complexities of polarization measurements in crystal samples for FTIR spectroscopy.

claywilliams
Download Presentation

Advanced Techniques in Linear Dichroism Spectrometry for Molecular Orientation Studies

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Polarization Modulation with PEM for CD (courtesy Hinds Instr.)

  2. Linear Dichroism Schematic(courtesy Hinds Instr.) Spectrometer UV or IR Linear polarizer PEM

  3. Variable angle of incidence Linear Dichroism - Reflection (IRRAS) (courtesy Hinds Instruments) Surface can be metal (high reflection, often grazing incidence) or dielectric, or even air-water interface For film on liquid use Langmuir-trough for sample--studies of peptides and proteins in membranes possible, orientation from polarization

  4. ATR Polarization Measurements IR beam multiply reflectsinside crystal -- penetrates surface keeps polarizations: Eperp in surface, Epara partially out In from FTIR E Crystal- Typ: Ge, ZnSe High index for refl. out Sample coated on crystal

  5. Schematic – External reflectance FTIR & L-B trough Dluhy, App.Spec.Rev.2000

  6. z E|| z B|| x B| E y x n = c/l l Light—Electro-Magnetic (E-M) radiation Tool for probing quantum mechanical states k|| y

More Related