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Lab session 5

Lab session 5. Separation of blood serum lipids by thin-layer chromatograghy. IUG, 2012 TMZ. Principle. The method is based on different migration rates of blood serum lipid fractions: Phospholipids Free fatty acids Cholesterol Glycerides

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Lab session 5

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  1. Lab session 5 Separation of blood serum lipids by thin-layer chromatograghy IUG, 2012 TMZ

  2. Principle • The method is based on different migration rates of blood serum lipid fractions: • Phospholipids • Free fatty acids • Cholesterol • Glycerides In a thin layer of adsorbent as an organic solvent moves through it.

  3. The rate of fractions separated is dependent upon their relative polarities. • A iodine vapor is then used to identify the separated fractions.

  4. Reagents & materials • Ethanol/diethyl ether mixture (3:1) • Chloroform • N-hexane/diethyl ether/acetic acid mixture (73:25:2) serves as a chromatography solvent • Metallic iodine • Thin lyer chromatography plates silufol UV254 • A chromatography chamber • A water bath • A development chamber • A chromatogram viewing cabinet • Blood serum sample

  5. Procedure 1. Mix 1 ml of blood serum with 10 ml of ethanol/ether mixture

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