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Objectives ??

Ex. 13: Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods. Objectives ?? . Vocabulary. Coliforms P ure culture Types of culture media: Selective Enrichment Differential UTI, Pyuria , Hematuria. Urinary Tract Infections (UTIs ).

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Objectives ??

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  1. Ex. 13: Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Objectives ??

  2. Vocabulary • Coliforms • Pure culture • Types of culture media: • Selective • Enrichment • Differential • UTI, Pyuria, Hematuria

  3. Urinary Tract Infections (UTIs) • Common? • Normal genitourinary flora? • Types of UTIs? • Significant bacteriuria in “clean catch” • Causative agents? • Diagnosis?

  4. EMB (Eosin Methylene Blue) Agar Contains peptones, lactose, sucrose, and the dyes eosin and methylene blue. Eosin dye inhibits growth of Gram+ bacteria. Methyleneblue acts as indicator. Lactose and sucrose are nutrients(fermentable carbohydrates)

  5. MacConkey Agar 1stsolid differential media. Developed at the turn of the 20th century by Alfred MacConkey, M.D. Contains peptones, lactose, bile salts and the dye crystal violet. Crystal violet and bile salts prevent growth of Gram+ and fastidious Gram – bacteria, such as Neisseria and Pasteurella. Lactose fermentation used to differentiate.

  6. Day 1

  7. Day 2 the end

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