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Go to the Reactants pane to fix pH and enable microbial kinetics.

Enter initial fluid composition on the Basis pane, then go to the Reactants pane. Choose microbial kinetics. Go to the Reactants pane to fix pH and enable microbial kinetics. . Enter microbial redox reaction. Enter thermodynamic constraints and kinetic parameters, including:

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Go to the Reactants pane to fix pH and enable microbial kinetics.

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  1. Enter initial fluid composition on the Basis pane, then go to the Reactants pane. Choose microbial kinetics Go to the Reactants pane to fix pH and enable microbial kinetics.

  2. Enter microbial redox reaction. Enter thermodynamic constraints and kinetic parameters, including: • rate constant • half-saturation constants • initial biomass • growth yield and decay constant • promoting and inhibiting species • ATP threshold energy

  3. Kinetics of microbially catalyzed reaction Rate is initially biomass limited, but increases until thermodynamic and kinetic limitations develop.

  4. Growth and decay of microbial populations Biomass increases rapidly, then slowly declines as decay outpaces growth

  5. Thermodynamic constraints on reaction Reaction proceeds uninhibited initially but rate slows after 40 hours primarily due to thermodynamic limitations.

  6. Identify each microbial population with a label and reaction. Microbes can compete for common substrates or carry out separate reactions. In this example, two different strains, (one fast, the other efficient) growing in a chemostat catalyze the same reaction. They differ in their kinetic and growth parameters.

  7. Pure cultures and mixed communities The rapidly metabolizing strain grows fastest initially, but with time is replaced by the more efficient strain.

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