Oncogenes, Tumor Suppressors, and the Cell Cycle

1. Oncogenes, Tumor Suppressors, and the Cell Cycle Radiobiology 2012

2. Cancer is consequence of abnormal increased cell growth and/or decreased cell death • Normal tissue homeostasis balances cell growth and death to maintain constant mass • Normal tissue hypertrophy can come from increased cell number (pregnant uterus), or increased cellular mass (exercising muscle) • Normal tissue regeneration can be physiologically necessary (bone marrow, gut epithelium, skin) or in response to damage (liver regeneration)

3. Cancer cell dynamics are not under normal processes of cell control • Oncogenes (activated proto-oncogenes) are generalized as drivers of tumor cell division • Tumor Suppressors are generalized as brakes to cellular division • Therefore oncogenic transformation combines activation of proto-oncogenes and loss of tumor suppressors to drive cell proliferation.

4. Impact of Transformation on the Cell Cycle

5. Many physical and chemical insults can activate a proto-oncogene

6. Several Molecular Mechanisms to activate a proto-oncogene

7. Cytogenetics identified the “Philadelphia Chromosome” Peter Nowell at Penn Found a small Ch22 in patients with CML

8. Identification of activated oncogenes experimentally H-ras V12D identified in 1983

9. Cell Fusion Identified the concept of “The Tumor Supressor Gene” Cancer Cell PEG or Virus Normal Cell Heterokaryon has more “Normal” Cellular phenotype Some normal gene was lost in cancer cell?

10. Tumor Suppressors require “Two-Hit” inactivation One allele may be lost in the germ line

11. LFS can result in many types of cancer

12. Cancer Predisposition Syndromes helped identify Tumor Suppressor Genes

13. Loss of Heterozygosity can Remove the Normal allele and result in functional loss

14. How do Oncogenes and tumor suppressor disrupt normal functions? • Ocogenes: • Stimulate the cell cycle (ras) • Inhibit death (Apoptotic) signalling (bcl2) • Drive metastatic growth (Met) • Tumor suppressors • Release brakes in the Cell Cycle (p53/Rb) • Release brakes on growth factor signals (PTEN) • Modulate response to stress (VHL) • Increased survival signals (APC)

15. Function of oncogenes in normal growth signal processes

16. Function of oncogenes in blocking normal death signals

17. MYC can drive several processes

18. Cancer is a multi-step process Colon Cancer MoleculrOncogenesis – Vogelstein and Kinzler

19. “Mutator” phenotype can accelerate Multi-Hits needed for Transformation DNA metabolism obiously important to maintain integrity of the genome

20. Metastatic spread requires additional genetic changes

21. Senescence can also block cellular transformation Normal fibroblasts Have finite number Of divisions before Permanent Arrest

22. Genomic Instability can accelerate Tumor formation • DNA damage can be: • Properly repaired without consequences • Non-repaired that can lead to cell death • Mis-repaired contributes to alterations in gene sequence or expression

23. Radiation Response impacts the Cell Cycle Machinery

24. G1 Arrest After XRT Deckbar et al Crit Rev Biochem 2011

25. G2/M arrest after XRT Deckbar et al Crit Rev Biochem 2011

26. DNA Repair–Deficiency can result in tumor predisposition

27. Loss of ATM predisposes to Radiation-induced Cancers

28. Mutations in Mismatch Repair genes causes Predisposition to CRC

29. Radiation also impacts Lipid Second Messenger Signaling Fuchs and Kolesnik model

30. NF-κB pathway is also Stress-Responsive Pro-Survival Signals Aid Tumor Cell Growth

31. Summary • Cancer is a multistep process • Gain of oncogenic function or loss of tumor suppressor function impacts many cellular processes (cell cycle, apoptosis, senescence) • Some genetic events that contribute to cancer formation can also influence response to DNA damage dependent therapy