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Transcription . Definitely not Transformation, Transduction or Translocation !. What is a Gene?. NOPE !. One Gene – One Enzyme One Gene – One Polypeptide One Gene – One RNA transcript A GENE IS A SEGMENT OF DNA THAT CODES FOR - - - - - - - - AN RNA TRANSCRIPT!!!!. NOPE !. YES !.

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  1. Transcription Definitely not Transformation, Transduction or Translocation!

  2. What is a Gene? NOPE ! • One Gene – One Enzyme • One Gene – One Polypeptide • One Gene – One RNA transcript • A GENE IS A SEGMENT OF DNA THAT CODES FOR - - - - - - - - AN RNA TRANSCRIPT!!!! NOPE ! YES !

  3. Which of the following will get translated? rRNA tRNA mRNA snRNA • A only • B only • C only • D only • A & B • B & C • C & D • A, B, & C • A, B, C, & D

  4. Which of the following are directly involved with translation? • A only • B only • C only • D only • A & B • B & C • C & D • A, B, & C • A, B, C, & D rRNA tRNA mRNA snRNA

  5. What best represents rRNA? • Red • Blue • Green • Brown

  6. What best represents tRNA? • Red • Blue • Green • Brown

  7. What best represents snRNA? • Red • Blue • Green • Brown

  8. Life is just an RNA SAMWICHH!

  9. Hey Check out this basic transcription animation! • http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2.htm l

  10. What did the idiot scribe represent? • RNA primase • RNA polymerase • DNA polymerase • Reverse transctriptase • Helicase

  11. What is/are the substrate (s) for RNA polymerase? • ATP, GTP, UTP, CTP • ATP only • dATP, dGTP, dCTP, dTTP • ATP, GTP, CTP, TTP • Amino acids

  12. How many RNA polymerases are there? • THREE • RNA Polymerase I • Transcribes nucleolar genes into rRNA • RNA Polymerase II • Transcribes genes into mRNA (which eventually turns into a polypeptide) • RNA Polymerase III • Transcribes genes into tRNA and snRNA

  13. How does an RNA Polymerase “FIND” a gene to transcribe? • TRANSCRIPTION FACTORS FIND A TATA BOX ON THE PROMOTER REGION!!!!!!!!!!!!!!!!!!!!

  14. Check Out the Process in a MovEEE • http://vcell.ndsu.edu/animations/transcription/index.htm

  15. What did Sir Spliceolot accomplish? • Cut out different DNA sections called introns. • Cut out different RNA sections called exons. • Cut out different RNA sections called introns. • Cut out different DNA sections called exons.

  16. Why are introns called introns? They are INTERVENING noncoding sequences found within a gene!

  17. Why are exons called exons? They are coding sections of a gene that get EXPRESSED as a component of a protein!

  18. What really splices out the introns? • Cutterase • Dicerase • Spliceosome • Blitzenase • Rudolphase

  19. What really is a SPLICEOSOME? small protein BIG PROTEIN snRNA snRNP SPLICEOSOME

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