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Edible Films. a nd Microbial Growth. Purpose of an Edible Film. Prevent Microorganisms from colonizing Seal for freshness Extend shelf life Inhibits loss of moisture Protect from handling. How an Edible Film is Produced. Apple Skin Polyphenols Wheat gluten and Gelatin
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Edible Films and Microbial Growth
Purpose of an Edible Film • Prevent Microorganisms from colonizing • Seal for freshness • Extend shelf life • Inhibits loss of moisture • Protect from handling
How an Edible Film is Produced • Apple Skin Polyphenols • Wheat gluten and Gelatin • Defatted mustard meal • Polysaccharides • Banana flour and Chitosan
What is an Edible Film applied to? • EVERYTHING! • Fruits • Vegetables • Meat • Fish
How do Edible Films on Food affect Microbial Growth? • Article 1: Bioactive alginate coatings to control Listeria monocytogenes on cold-smoked salmon slices and fillets • Article 2: Physical and antimicrobial properties of banana flour/chitosan biodegradable and self sealing films used for preserving Fresh-cut vegetables
Article 1: Salmon Experiment • Published: From the University of Delaware, Newark, DE Available online 13 October 2009. • The experiment: • Five L. monocytogenes strains, PSU1, PSU9, F5069, ATCC 19115 and Scott A • Used to inoculate salmon pieces.
Article 1: Salmon Experiment • Methods: • Slices of smoked salmon were punched aseptically into 5.7-cm diameter round pieces (~10g) and surface-inoculated with 125 µl L. monocytogenes • Five antimicrobials: alginate, κ-carrageenan, pectin, starch and gelatin • Stored for 6 days
Article 1: Salmon Experiment • Results: Compared to the control, all antimicrobial coatings were successful. Alginate coating was most effective
Article 2: Fresh Cut Vegetables • Published: Asian Institute of Technology, KlongLuang, Pathumthani 12120, Thailand Available online 15 June 2011. • The experiment: • Fresh vegetables (asparagus, baby corn, oyster mushroom and Chinese cabbage) • Find changes in the number of S. aureus with and without a film sealed bag
Article 2: Fresh Cut Vegetables • Methods: • Cut fresh vegetables and placed in bags. • 3 days in the refrigerator • Checked for S. aureus growth
Article 2: Fresh Cut Vegetables • Results: • Within 3 days- reduced levels on asparagus, baby corn, oyster mushrooms, and Chinese cabbage • Mushrooms were found to be same as control • chitosan restricts the available nutrients to the bacterial cells, also it has the limits water and gas transfer conditions
Applications to Dietetic Practice • Allergy to edible film • Those prone to sickness
Applications to Dietetic Practice • Improve safety and quality • Longer shelf life (If busy or on a budget)