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A. B. lys. sup. ppt. c. a. b. lys. ppt. lys. ppt. lys. ppt. Supplemental Figure 1
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A B lys sup ppt c a b lys ppt lys ppt lys ppt Supplemental Figure 1 Immunoblotting analysis of PECAM1, PLVAP, and vWf in immunoprecipitate from protein lysate from kidney of blood type A using anti-blood type A antibody. In panel A, lysate was subjected to immunoprecipitation using anti-type A mouse monoclonal antibody in combination with rat anti-mouse IgM antibody conjugated to Sepharose CL-4B (04-6841, Invitrogen, Carisbad, CA, USA). All the immunoreactivity with anti-A antibody detected in lysate (lys) was disappeared from supernatant (sup), and successfully recovered in immunoprecipitate (ppt). In panel B, both lysate (lys) and immunoprecipitate (ppt) were analyzed with immunoblotting using anti-PECAM1 (a), anti-PLVAP (b) or anti-vWf (c). PECAM1 PLVAP vWf