1 / 24

How will you know what your experimental data means?

How will you know what your experimental data means?. Student’s Lab Notebook. Making Sense of Your Data. What data did you take? What are some things your data requires you to understand?. Can difference in color be due to genes?. What is known about energy production in Halo?. ATP.

coye
Download Presentation

How will you know what your experimental data means?

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. How will you know what your experimental data means? Student’s Lab Notebook

  2. Making Sense of Your Data • What data did you take? • What are some things your data requires you to understand?

  3. Can difference in color be due to genes?

  4. What is known about energy production in Halo?

  5. ATP • Cells need ATP for nearly all cellular processes that require energy, including growth • Therefore, halo must be able to make ATP in the conditions that allow them to grow

  6. P P P P P P MORE stored energy ATP OUTPUT INPUT ENERGY FOR CELLULAR WORK CHEMICAL ENERGY + P ADP LESS stored energy

  7. Question #1 What makes halobacterium purple?

  8. Halo have a purple membrane protein called bacteriorhodopsin (bR) Halobacterium cell Bacteriorhodopsin (bR)

  9. Halo have a purple membrane protein called bacteriorhodopsin (bR) Halobacterium cell Bacteriorhodopsin (bR)

  10. Question #2 What is the purple membrane protein made of?

  11. Bacteriorhodopsin (bR) Halo have a purple membrane protein called bacteriorhodopsin (bR) Cell membrane

  12. Retinal Bacteriorhodopsin (bR) is made from a protein called bop and the molecule retinal Bop + retinal = bacteriorhodopsin (bR) Cell membrane Bacteriorhodopsin (bR) Bop protein

  13. Question #3 What is the purpose of the purple membrane protein?

  14. BR converts light energy into chemical energy for making ATP LIGHT Halobacterium cell ATP ATP ATP ATP ATP Bacteriorhodopsin (bR) Note: other proteins are required for this process, but we will simplify and focus on bR

  15. BR converts light energy into chemical energy for making ATP LIGHT Halobacterium cell ATP ATP ATP ATP ATP Bacteriorhodopsin (BR) Note: other proteins are required for this process, but we will simplify and focus on BR

  16. Bacteriorhodopsin (bR) BR converts light energy into chemical energy for making ATP Cell membrane LIGHT ADP + P ATP

  17. Bacteriorhodopsin (bR) ADP + P BR converts light energy into chemical energy for making ATP LIGHT PRESENT Cell membrane LIGHT ATP ATP

  18. Halo use bacteriorhodopsin (bR) to produce ATP using light energy LIGHT PRESENT LIGHT ABSENT (DARK) LIGHT LIGHT Bacteriorhodopsin (bR) Bacteriorhodopsin (bR) ADP ADP + + P P ATP ATP

  19. Halobacterium cell Halobacterium cell Halo use bacteriorhodopsin (bR) to produce ATP using light energy LIGHT PRESENT LIGHT ABSENT (DARK) LIGHT LIGHT ATP ATP ATP ATP ATP ATP ATP ATP ATP ATP Halo use bR to produce ATP for growth and metabolism Halo cannot use bR to produce ATP

  20. Question #4 How would amount of light and levels of purple protein be connected?

  21. Halo change the expression of bR in response to the amount of light in their environment LIGHT Halobacterium cell Bacteriorhodopsin (bR)

  22. Halo change the expression of bR in response to the amount of light in their environment LIGHT Halobacterium cell Bacteriorhodopsin (bR)

  23. Halobacterium cell Halobacterium cell Halo change the expression of bR in response to the amount of light in their environment LIGHT PRESENT LIGHT ABSENT (DARK) LIGHT LIGHT When there is more light, halo respond by making more bR When there is less light, halo make less bR

  24. OUR QUESTION: How do Halobacterium cells control the amount of bR expressed in response to light? What is the gene and protein network that regulates the expression of bR?

More Related