1 / 17

Immunology Chapter 5, Lecture 1

Immunology Chapter 5, Lecture 1. Richard L. Myers, Ph.D. Department of Biology Southwest Missouri State Temple Hall 227 Telephone: 417-836-5307 Email: rlm967f@mail.smsu.edu Homepage: http://creative.smsu.edu/biology/myersr/index.html TopClass: http://creative.smsu.edu.

curran-horn
Download Presentation

Immunology Chapter 5, Lecture 1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ImmunologyChapter 5, Lecture 1 • Richard L. Myers, Ph.D. • Department of Biology • Southwest Missouri State • Temple Hall 227 • Telephone: 417-836-5307 • Email: rlm967f@mail.smsu.edu • Homepage: http://creative.smsu.edu/biology/myersr/index.html • TopClass: http://creative.smsu.edu

  2. Basic structure of Ab • Antibodies (Ab) are found in the serum • Four fractions seen after electrophoresis (Tiselius and Kabat): • albumin • alpha (a) • beta (b) • gamma (g) • After reaction of hyperimmune serum with antigen, the gamma fraction is reduced

  3. Experiments to show Ig structure • Porter • used brief papain digestion to produce fragments • produced two identical fragments • called Fab because they bound antigen • MW of 45, 000 • produced a larger fragment • called Fc because it could be crystallized • MW of 50,000

  4. Experiments (cont) • Edelman • reduced the interchain disulfide bonds with ethanol and alkylation • followed by gel filtration • produced two peaks • Peak I contained the 50,000 MW heavy chain • Peak II contained the 25,000 MW light chain

  5. Fine structure of Ig • Bence-Jones provided early information about Ig structure • Light chain structure • variable (V) region • constant (C) region • contains kappa (k) or lambda (l) markers • 60% kappa, 40% lambda in humans • a single ab molecule expresses only one of these

  6. Fine structure (cont) • Heavy chain • contains a variable (V) region • also contains a constant (C) region • can be disignated as m, g, a, d or e • each represents a different class of Ab • Domains of heavy and light chains interact to specifically bind antigen and play some effector roles

  7. Variable region domains • Amino acid composition of VH and VL gives information about Ab specificity • Sequence variability is concentrated in hypervariable regions • Three hypervariable regions found in both heavy and light chain • Form the basis for antibody specificity • Complementarity-determining regions

  8. IgG animation

  9. Assignment • Read Chapter 5, Immunoglobulins: Structure and Function • Review question 2 (pg 141)

More Related